Lai Xiao, Niroula Dhirendra, Burrows Mary, Wu Xiaogang, Yan Qing
Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA.
College of Agriculture, Guangxi University, Nanning 530004, China.
Microorganisms. 2022 Aug 8;10(8):1596. doi: 10.3390/microorganisms10081596.
Antibiosis has been proposed to contribute to the beneficial bacteria-mediated biocontrol against pea Aphanomyces root rot caused by the oomycete pathogen . However, the antibiotics required for disease suppression remain unknown. In this study, we found that the wild type strains of Pf-5 and 2P24, but not their mutants that lack 2,4-diacetylphloroglucinol, strongly inhibited on culture plates. Purified 2,4-diacetylphloroglucinol compound caused extensive hyphal branching and stunted hyphal growth of . Using a GFP-based transcriptional reporter assay, we found that expression of the 2,4-diacetylphloroglucinol biosynthesis gene is activated by germinating pea seeds. The 2,4-diacetylphloroglucinol producing Pf-5 derivative, but not its 2,4-diacetylphloroglucinol non-producing mutant, reduced disease severity caused by on pea plants in greenhouse conditions. This is the first report that 2,4-diacetylphloroglucinol produced by strains of species plays an important role in the biocontrol of pea Aphanomyces root rot.
有人提出，抗生作用有助于有益细菌介导的对由卵菌病原体引起的豌豆瓜果腐霉菌根腐病的生物防治。然而，抑制该病所需的抗生素仍不清楚。在本研究中，我们发现Pf-5和2P24的野生型菌株，而非缺乏2,4-二乙酰基间苯三酚的突变体，在培养平板上有强烈抑制作用。纯化的2,4-二乙酰基间苯三酚化合物导致瓜果腐霉菌丝大量分支并生长受阻。使用基于绿色荧光蛋白的转录报告分析，我们发现2,4-二乙酰基间苯三酚生物合成基因的表达在豌豆种子萌发时被激活。在温室条件下，产生2,4-二乙酰基间苯三酚的Pf-5衍生物，而非不产生2,4-二乙酰基间苯三酚的突变体，降低了豌豆植株上由瓜果腐霉引起的病害严重程度。这是关于某种物种菌株产生的2,4-二乙酰基间苯三酚在豌豆瓜果腐霉菌根腐病生物防治中起重要作用的首次报道。
