Legrifi Ikram, Al Figuigui Jamila, El Hamss Hajar, Lazraq Abderrahim, Belabess Zineb, Tahiri Abdessalem, Amiri Said, Barka Essaid Ait, Lahlali Rachid
Phytopathology Unit, Department of Plant Protection, Ecole Nationale d'Agriculture de Meknès, Km 10, Rte Haj Kaddour, BP S/40, Meknès 50001, Morocco.
Laboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Route d'Imouzzer, Fez 30000, Morocco.
Microorganisms. 2022 Aug 12;10(8):1635. doi: 10.3390/microorganisms10081635.
Several diseases affect the productivity of olive trees, including root rot disease caused by genera. Chemical fungicides, which are often used to manage this disease, have harmful side effects on humans as well as environmental components. Biological management is a promising control approach that has shown its great potential as an efficient eco-friendly alternative to treating root rot diseases. In the present study, the antagonistic activity of ten bacterial isolates was tested both in vitro and in planta against , the causal agent of olive root rot disease. These bacterial isolates belonging to the genera , , , , and were chosen for their potential antimicrobial effects against many pathogens. Results of the in vitro confrontation bioassay revealed a high reduction of mycelial growth exceeding 80%. The antifungal effect of the volatile organic compounds (VOCs) was observed for all the isolates, with mycelial inhibition rates ranging from 28.37 to 70.32%. Likewise, the bacterial cell-free filtrates showed important inhibition of the mycelial growth of the pathogen. Overall, their efficacy was substantially affected by the nature of the bacterial strains and their modes of action. A greenhouse test was then carried out to validate the in vitro results. Interestingly, two bacterial isolates, ACBC1 and SF14, were the most successful in managing the disease. Our findings suggested that these two antagonistic bacterial isolates have promising potential as biocontrol agents of olive root rot disease.
几种病害会影响橄榄树的产量，包括由多种病原菌引起的根腐病。化学杀菌剂常用于防治这种病害，但对人类和环境成分都有有害副作用。生物防治是一种很有前景的防治方法，已显示出作为治疗根腐病的高效环保替代方法的巨大潜力。在本研究中，测试了10株细菌分离株对橄榄根腐病病原菌在体外和植物体内的拮抗活性。这些属于芽孢杆菌属、假单胞菌属、伯克霍尔德氏菌属、沙雷氏菌属和肠杆菌属的细菌分离株因其对多种病原体的潜在抗菌作用而被选中。体外对峙生物测定结果显示，菌丝体生长显著减少，超过80%。观察到所有分离株的挥发性有机化合物（VOCs）都有抗真菌作用，菌丝体抑制率在28.37%至70.32%之间。同样，细菌无细胞滤液对病原菌的菌丝体生长也有重要抑制作用。总体而言，它们的功效受细菌菌株的性质及其作用方式的影响很大。随后进行了温室试验以验证体外试验结果。有趣的是，两株细菌分离株，芽孢杆菌ACBC1和假单胞菌SF14，在病害防治方面最为成功。我们的研究结果表明，这两种拮抗细菌分离株作为橄榄根腐病的生物防治剂具有广阔的潜力。
