Tian Peng, He Liang, Guo Xiaoyi, Ma Zeyu, Song Ruiqi, Liao Xiaoqiao, Gan Fangji
School of Mechanical Engineering, Sichuan University, Chengdu 610065, China.
Micromachines (Basel). 2022 Aug 9;13(8):1284. doi: 10.3390/mi13081284.
The aspheric light emitted from a pinhole restrains the reconstruction quality of a digital in-line hologram. Herein, the Fresnel-diffracted spot from the first step converging spherical wave diffracted at a rough circular aperture is collimated and expanded to generate an even plane wave, which is converged again by an objective lens and matching a minimum aperture while the central spot is varying from light to dark. We observed that the collected background hologram is filled with a round spot with high contrast as an ideal spherical wave. The resolution board and biology experimental results demonstrated a distinctively reconstructed image without any image processing in a single exposure. The adjustable field of view and magnification, single exposure, and noncontact make it suitable for an online microscope.
针孔发出的非球面光会限制数字同轴全息图的重建质量。在此，第一步在粗糙圆孔处衍射的会聚球面波产生的菲涅耳衍射光斑被准直并扩展，以产生均匀平面波，该平面波由物镜再次会聚，并与最小孔径匹配，同时中心光斑从亮变暗。我们观察到，采集到的背景全息图充满了一个高对比度的圆形光斑，作为理想的球面波。分辨率板和生物学实验结果表明，在单次曝光且无需任何图像处理的情况下，重建图像清晰独特。其可调的视野和放大倍数、单次曝光以及非接触特性使其适用于在线显微镜。
