Gorniak T, Heine R, Mancuso A P, Staier F, Christophis C, Pettitt M E, Sakdinawat A, Treusch R, Guerassimova N, Feldhaus J, Gutt C, Grübel G, Eisebitt S, Beyer A, Gölzhäuser A, Weckert E, Grunze M, Vartanyants I A, Rosenhahn A
Applied Physical Chemistry, University of Heidelberg, Heidelberg, Germany.
Opt Express. 2011 Jun 6;19(12):11059-70. doi: 10.1364/OE.19.011059.
The imaging of hydrated biological samples - especially in the energy window of 284-540 eV, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. Free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. One current challenge is to match both the desired energy and the intensity of the light source. We present the first images of dehydrated biological material acquired with 3rd harmonic radiation from FLASH by digital in-line zone plate holography as one step towards the vision of imaging hydrated biological material with photons in the water window. We also demonstrate the first application of ultrathin molecular sheets as suitable substrates for future free-electron laser experiments with biological samples in the form of a rat fibroblast cell and marine biofouling bacteria Cobetia marina.
水合生物样品的成像——尤其是在284 - 540电子伏特的能量窗口中，在这个能量窗口里水不会掩盖软有机物质和生物相关元素的信号——对生命科学具有极大的吸引力。自由电子激光可以提供高强度且相干的脉冲，这使得单脉冲成像能够克服由辐射损伤所设定的分辨率限制。当前的一个挑战是要使光源的能量和强度都符合要求。我们展示了通过数字在线波带片全息术利用FLASH的三次谐波辐射获取的脱水生物材料的首批图像，这是朝着利用水窗中的光子对水合生物材料进行成像这一愿景迈出的一步。我们还展示了超薄分子片作为未来以大鼠成纤维细胞和海洋生物污损细菌滨海考贝氏菌形式的生物样品进行自由电子激光实验的合适基底的首次应用。
