School of Food and Drug, Luoyang Normal University, Luoyang 471934, China.
Beijing Academy of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Molecules. 2022 Aug 19;27(16):5297. doi: 10.3390/molecules27165297.
Soybean is widely used as a kind of bean for daily consumption. Chickpea is increasingly utilised because of its good healthcare function. At present, using chickpeas could have better results than soybeans in some areas. Previous studies of the two legumes focused on certain components and failed to fully reveal the differences between the two legumes. Thus, understanding the comprehensive similarities and differences between the two legumes is necessary to apply and develop these legumes effectively. In this study, we performed a UPLC-ESI-MS/MS-based widely targeted metabolomics analysis on two legumes. A total of 776 metabolites (including primary metabolites and secondary metabolites) were detected, which were divided into more than a dozen broad categories. The differential analysis of these metabolites showed that there were 480 metabolites with significant differences in relative contents between the two legumes. Compared with soybean, the expression of 374 metabolites of chickpea was down-regulated and that of 106 metabolites was up-regulated. The metabolic pathway analysis showed significant differences in the flavonoids biosynthesis, phenylpropanoid biosynthesis, linoleic acid metabolism and alkaloid biosynthesis between the two legumes. The advantages and applicability of the two kinds of legumes were confirmed through the analysis of anti-diabetic components. Moreover, some novel compounds (with contents higher than that of soybean) with hypoglycaemic activity were found in chickpea. This study provides an important reference for the in-depth study and comparative application of soybean and chickpea.
大豆被广泛用作日常消费的豆类。由于其良好的保健功能，鹰嘴豆的应用也越来越广泛。目前，在某些地区，鹰嘴豆的使用可能比大豆效果更好。以前对这两种豆类的研究集中在某些成分上，未能充分揭示它们之间的差异。因此，了解这两种豆类的综合相似性和差异性，对于有效地应用和开发这些豆类是必要的。在这项研究中，我们对两种豆类进行了基于 UPLC-ESI-MS/MS 的广泛靶向代谢组学分析。共检测到 776 种代谢物（包括初级代谢物和次级代谢物），分为十几个大类。这些代谢物的差异分析表明，两种豆类之间有 480 种代谢物的相对含量有显著差异。与大豆相比，鹰嘴豆中有 374 种代谢物的表达下调，106 种代谢物的表达上调。代谢途径分析表明，两种豆类在类黄酮生物合成、苯丙素生物合成、亚油酸代谢和生物碱生物合成方面存在显著差异。通过对降糖成分的分析，证实了两种豆类的优势和适用性。此外，在鹰嘴豆中还发现了一些含量高于大豆的具有降血糖活性的新型化合物。本研究为深入研究和比较大豆和鹰嘴豆的应用提供了重要参考。
