Department of Pediatric Surgery, Affiliated Hospital of Jining Medical University, Jining 272067, China.
College of Pharmacy, Heze University, Heze 274015, China.
Molecules. 2022 Aug 22;27(16):5337. doi: 10.3390/molecules27165337.
As one of marine tetrahydroisoquinoline alkaloids, renieramycin T plays a significant role in inhibiting tumor metastasis and invasion. However, the effect of renieramycin T on inflammation-related tumor metastasis and invasion is still unknown, and its mechanisms remain unclear. Here we established an inflammation-related tumor model by using the supernatant of RAW264.7 cells to simulate B16F10 mouse melanoma cells. The results indicate that renieramycin T suppressed RAW264.7 cell supernatant-reduced B16F10 cell adhesion to a fibronectin-coated substrate, migration, and invasion through the matrigel in a concentration-dependent manner. Moreover, Western blot results reveal that renieramycin T attenuated the phosphorylation of STAT3 and down-regulated the expression of Nrf2. Together, the above findings suggest a model of renieramycin T in suppressing B16F10 cancer cell migration and invasion. It may serve as a promising drug for the treatment of cancer metastasis.
作为海洋四氢异喹啉生物碱之一，雷尼替丁 T 在抑制肿瘤转移和侵袭方面发挥着重要作用。然而，雷尼替丁 T 对炎症相关肿瘤转移和侵袭的影响尚不清楚，其机制也尚不清楚。在这里，我们通过使用 RAW264.7 细胞的上清液来模拟 B16F10 小鼠黑色素瘤细胞，建立了一个炎症相关的肿瘤模型。结果表明，雷尼替丁 T 能够浓度依赖性地抑制 RAW264.7 细胞上清液减少的 B16F10 细胞对纤连蛋白包被基底的黏附、迁移和侵袭。此外，Western blot 结果表明，雷尼替丁 T 减弱了 STAT3 的磷酸化，并下调了 Nrf2 的表达。综上所述，这些发现提出了雷尼替丁 T 抑制 B16F10 癌细胞迁移和侵袭的模型。它可能成为治疗癌症转移的一种有前途的药物。
