Istituto Zooprofilattico Sperimentale delle Venezie, Department of Chemistry, viale dell'Università, 35020 Padova, Italy.
Department of Biomedical Sciences, University of Padova, via Ugo Bassi 58/b, 35131 Padova, Italy.
Nutrients. 2022 Aug 11;14(16):3288. doi: 10.3390/nu14163288.
Hypertension is the leading risk factor for premature death worldwide and significantly contributes to the development of all major cardiovascular disease events. The management of high blood pressure includes lifestyle changes and treatment with antihypertensive drugs. Recently, it was demonstrated that a diet supplemented with (TM) extracts is useful in the management of numerous pathologies, including hypertension. This study is aimed at unveiling the underlying mechanism and the molecular targets of intervention of TM dietary supplementation in hypertension treatment by means of proteomics and metabolomics techniques based on liquid chromatography coupled with high-resolution mass spectrometry. We demonstrate that serum proteome and metabolome of spontaneously hypertensive rats are severely altered with respect to their normotensive counterparts. Additionally, our results reveal that a diet enriched with TM extracts restores the expression of 15 metabolites and 17 proteins mainly involved in biological pathways associated with blood pressure maintenance, such as the renin-angiotensin and kallikrein-kinin systems, serin protease inhibitors, reactive oxygen scavenging, and lipid peroxidation. This study provides novel insights into the molecular pathways that may underlie the beneficial effects of TM, thus corroborating that TM could be proposed as a helpful functional food supplement in the treatment of hypertension.
高血压是全球导致过早死亡的主要风险因素，也是所有主要心血管疾病事件发生的重要原因。高血压的治疗包括生活方式的改变和抗高血压药物的治疗。最近，研究表明，补充（TM）提取物的饮食对包括高血压在内的多种疾病的治疗有用。本研究旨在通过基于液相色谱-高分辨质谱联用的蛋白质组学和代谢组学技术，揭示 TM 饮食补充治疗高血压的潜在机制和干预的分子靶点。我们证明，与正常血压大鼠相比，自发性高血压大鼠的血清蛋白质组和代谢组发生了严重改变。此外，我们的结果表明，富含 TM 提取物的饮食可恢复 15 种代谢物和 17 种蛋白质的表达，这些代谢物和蛋白质主要涉及与血压维持相关的生物学途径，如肾素-血管紧张素和激肽-激肽系统、丝氨酸蛋白酶抑制剂、活性氧清除和脂质过氧化。这项研究为 TM 可能产生有益影响的分子途径提供了新的见解，从而证实 TM 可作为治疗高血压的有用功能性食品补充剂。
