Di Giacomo Silvia, Di Sotto Antonella, Angelis Apostolis, Percaccio Ester, Vitalone Annabella, Gullì Marco, Macone Alberto, Axiotis Evangelos, Skaltsounis Alexios Leandros
Department of Physiology and Pharmacology "V. Erspamer", Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Pharmaceuticals (Basel). 2022 Aug 11;15(8):987. doi: 10.3390/ph15080987.
Boiss. (Fam. Lamiaceae) is an endemic plant of the North Aegean Islands (Greece), commonly known as ironwort. Traditionally, its aerial parts have been used to relieve several ailments, especially gastrointestinal disorders, however, with scant knowledge about the pharmacological basis. In the present study, an endemic Greek species from Lesvos Island has been characterized for phytochemical composition and biological activities, in order to give a possible scientific basis to its traditional use and to highlight a further nutraceutical interest as a source of bioactive phytochemicals and extracts. Three different fractions obtained from a methanolic extract of aerial parts by using ethyl acetate with 10 (S10), 20 (S20), and 50% (S50) methanol as fractionation solvents were phytochemically characterized. Moreover, their antioxidant power and cytoprotective activity in different human cell lines were evaluated. The phytochemical analysis highlighted the presence of flavonoids, iridoids, and phenolic acids in all the tested samples. Particularly, the S10 fraction mainly contained iridoids, while S20 and S50 lavandulifolioside and chlorogenic acid, respectively. The fractions also showed antioxidant properties, S10 and S20 being the most potent. When assessed in human cholangiocytes, they counteracted the cytotoxicity of the tBOOH pro-oxidant agent, by reducing ROS levels and affecting GSH antioxidant system. The present findings highlight a possible interest in S10 and S20 fractions from as sources of bioactive molecules and stimulate further studies in order to characterize their possible application for nutraceutical and pharmaceutical purposes.
博伊斯（唇形科）是北爱琴海诸岛（希腊）的一种特有植物，俗称铁草。传统上，其地上部分被用于缓解多种疾病，尤其是胃肠道疾病，然而，对其药理基础知之甚少。在本研究中，对一种来自莱斯博斯岛的希腊特有物种进行了植物化学成分和生物活性的表征，以便为其传统用途提供可能的科学依据，并突出其作为生物活性植物化学物质和提取物来源的进一步营养保健价值。使用乙酸乙酯与10%（S10）、20%（S20）和50%（S50）甲醇作为分馏溶剂，从地上部分的甲醇提取物中获得的三种不同馏分进行了植物化学表征。此外，还评估了它们在不同人类细胞系中的抗氧化能力和细胞保护活性。植物化学分析表明，所有测试样品中均存在黄酮类化合物、环烯醚萜类化合物和酚酸。特别是，S10馏分主要含有环烯醚萜类化合物，而S20和S50分别含有薰衣草苷和绿原酸。这些馏分还表现出抗氧化特性，S10和S20最为有效。在人胆管细胞中评估时，它们通过降低ROS水平和影响GSH抗氧化系统来抵消tBOOH促氧化剂的细胞毒性。本研究结果突出了对来自[植物名称未给出]的S10和S20馏分作为生物活性分子来源的潜在兴趣，并刺激进一步研究以表征它们在营养保健和制药目的方面的可能应用。
