Sarikurkcu Cengiz, Locatelli Marcello, Mocan Andrei, Zengin Gokhan, Kirkan Bulent
Department of Analytical Chemistry, Afyonkarahisar University of Health Sciences, Faculty of Pharmacy, Afyonkarahisar, Turkey.
Department of Pharmacy, D'Annunzio University of Chieti-Pescara, Chieti, Italy.
Front Pharmacol. 2020 Feb 14;10:1642. doi: 10.3389/fphar.2019.01642. eCollection 2019.
, also named "ironwort," "mountain tea," or "shepherd's tea," is a genus of flowering plants used as herbal medicine in traditional Mediterranean-area medicine systems, and these plants are generally consumed as a herbal tea. Its use as herbal tea and in traditional herbal medicine is quite popular. There are currently few studies on L., and only one reports the use of a liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MS) profile and the content of phenolic compounds without considering a possible correlation with its biological activities. This paper aims to investigate the antioxidant activities by means of several different biological/biochemical assays (radical scavenging, reducing power, ferrous ion chelating, and total antioxidant by phosphomolybdenum and β-carotene bleaching methods) as well as analyze the enzyme inhibitory activities (against AChE (acetylcholinesterase), BChE (butyrylcholinesterase), tyrosinase, α-glucosidase, and α-amylase) as well as the total phenolics, flavonoids, and condensed tannins. The reported results on highlighted that methanol and water extracts generally showed higher radical scavenging and reducing power activities. A similar trend could be observed for phosphomolybdenum and ferrous ion chelating activities. Methanol extracts showed lower activity only for the β-carotene bleaching assay.
，也被称为“铁草”“山茶”或“羊倌茶”，是一类开花植物，在传统地中海地区医学体系中用作草药，这些植物通常被当作花草茶饮用。它作为花草茶和用于传统草药非常受欢迎。目前关于 的研究很少，只有一篇报道了采用液相色谱-二极管阵列检测-电喷雾电离串联质谱（LC-DAD-ESI-MS）分析其酚类化合物的图谱和含量，但未考虑其与生物活性的可能相关性。本文旨在通过几种不同的生物/生化测定方法（自由基清除、还原能力、亚铁离子螯合以及磷钼酸和β-胡萝卜素漂白法测定总抗氧化能力）研究其抗氧化活性，同时分析其酶抑制活性（针对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、酪氨酸酶、α-葡萄糖苷酶和α-淀粉酶）以及总酚、黄酮和缩合单宁的含量。关于 的报道结果表明，甲醇提取物和水提取物通常表现出较高的自由基清除和还原能力活性。磷钼酸和亚铁离子螯合活性也观察到类似趋势。甲醇提取物仅在β-胡萝卜素漂白试验中表现出较低活性。
