IMEC, 3000 Leuven, Belgium.
Faculty of Engineering Science, Katholieke Universiteit Leuven (KUL), 3000 Leuven, Belgium.
Sensors (Basel). 2022 Aug 13;22(16):6059. doi: 10.3390/s22166059.
Background: Although both speckle plethysmography (SPG) and photoplethysmography (PPG) examine pulsatile changes in the vasculature using opto-electronics, PPG has a long history, whereas SPG is relatively new and less explored. The aim of this study was to compare the effects of integration time and light-source coherence on signal quality and waveform morphology for reflective and transmissive rSPG and rPPG. Methods: (A) Using time-domain multiplexing, we illuminated 10 human index fingers with pulsed lasers versus LEDs (both at 639 and 850 nm), in transmissive versus reflective mode. A synchronized camera (Basler acA2000-340 km, 25 cm distance, 200 fps) captured and demultiplexed four video channels (50 fps/channel) in four stages defined by illumination mode. From all video channels, we derived rPPG and rSPG, and applied a signal quality index (SQI, scale: Good > 0.95; Medium 0.95−0.85; Low 0.85−0.8; Negligible < 0.8); (B) For transmission videos only, we additionally calculated the intensity threshold area (ITA), as the area of the imaging exceeding a certain intensity value and used linear regression analysis to understand unexpected similarities between rPPG and rSPG. Results: All mean SQI-values. Reflective mode: Laser-rSPG > 0.965, LED-rSPG < 0.78, rPPG < 0.845. Transmissive mode: 0.853−0.989 for rSPG and rPPG at all illumination settings. Coherent mode: Reflective rSPG > 0.951, reflective rPPG < 0.740, transmissive rSPG and rPPG 0.990−0.898. Incoherent mode: Reflective all <0.798 and transmissive all 0.92−0.987. Linear regressions revealed similar R2 values of rPPG with rSPG (R2 = 0.99) and ITA (R2 = 0.98); Discussion: Laser-rSPG and LED-rPPG produced different waveforms in reflection, but not in transmission. We created the concept of ITA to investigate this behavior. Conclusions: Reflective Laser-SPG truly originated from coherence. Transmissive Laser-rSPG showed a loss of speckles, accompanied by waveform changes towards rPPG. Diffuse spatial intensity modulation polluted spatial-mode SPG.
尽管光体积描记术(SPG)和光电容积描记术(PPG)都使用光电技术检查血管的脉动变化,但 PPG 历史悠久,而 SPG 相对较新且探索较少。本研究旨在比较积分时间和光源相干性对反射和透射 rSPG 和 rPPG 的信号质量和波形形态的影响。
(A)使用时域复用,我们用脉冲激光和 LED(均为 639nm 和 850nm)照射 10 个人类食指,以透射和反射模式照射。同步摄像机(Basler acA2000-340km,25cm 距离,200fps)以四个阶段捕捉和多路分解四个视频通道(每个通道 50fps),这些阶段由照明模式定义。从所有视频通道中,我们得出 rPPG 和 rSPG,并应用信号质量指数(SQI,范围:好>0.95;中等 0.95-0.85;低 0.85-0.8;可忽略<0.8);(B)仅对传输视频,我们还计算了强度阈值面积(ITA),即超过一定强度值的成像面积,并使用线性回归分析来理解 rPPG 和 rSPG 之间意想不到的相似性。
所有平均 SQI 值。反射模式:激光-rSPG>0.965,LED-rSPG<0.78,rPPG<0.845。透射模式:所有照明设置下 rSPG 和 rPPG 的 0.853-0.989。相干模式:反射性 rSPG>0.951,反射性 rPPG<0.740,透射性 rSPG 和 rPPG 0.990-0.898。非相干模式:反射性均<0.798,透射性均 0.92-0.987。线性回归显示 rPPG 与 rSPG(R2=0.99)和 ITA(R2=0.98)的相似 R2 值。
激光-rSPG 和 LED-rPPG 在反射中产生不同的波形,但在透射中没有。我们创建了 ITA 的概念来研究这种行为。
反射性激光-SPG 确实源于相干性。透射性激光-rSPG 显示斑点丢失,同时波形向 rPPG 变化。漫射空间强度调制污染了空间模式 SPG。