缬沙坦对糖尿病肾病大鼠 LN、FN、MDA、肾组织纤维化及炎症浸润的影响。
Effects of Valsartan on LN, FN, MDA, Renal Tissue Fibrosis, and Inflammatory Infiltration in DN Rats.
机构信息
Department of Basic Medica, Jiangsu College of Nursing, Huaian 223005, China.
Department of Endocrinology, Huai'an First People's Hospital, Huaian 223300, China.
出版信息
Contrast Media Mol Imaging. 2022 Aug 4;2022:6473393. doi: 10.1155/2022/6473393. eCollection 2022.
The effects of valsartan on laminin (LN), fibronectin (FN), malondialdehyde (MDA), renal tissue fibrosis, and inflammatory infiltration in diabetic nephropathy (DN) rats are explored. A total of 42 SPF male Sprague D (SD) rats are selected and randomly divided into normal set, model set, valsartan low-dose and high-dose sets, and metformin set with 7 rats in each set. The kidney tissue of all rats is collected after administration. The standard of protein mRNA in kidney tissues is detected by real-time fluorescence quantitative polymerase chain reaction (PCR) method, and the protein standard in kidney tissues is detected by western blot. The experimental results show that the application of valsartan to DN rats can effectively relieve the morphology of the rat kidney tissue, enhance the protein expression in the kidney tissue of the DN rats, and reduce the fibrosis and inflammatory infiltration of the kidney tissue.
探讨缬沙坦对糖尿病肾病（DN）大鼠层粘连蛋白（LN）、纤维连接蛋白（FN）、丙二醛（MDA）、肾组织纤维化和炎症浸润的影响。选择 42 只 SPF 雄性 Sprague D（SD）大鼠，随机分为正常组、模型组、缬沙坦低剂量组和高剂量组、二甲双胍组，每组 7 只。给药后采集所有大鼠的肾组织。采用实时荧光定量聚合酶链反应（PCR）法检测肾组织中蛋白 mRNA 的标准，采用 Western blot 检测肾组织中蛋白的标准。实验结果表明，缬沙坦应用于 DN 大鼠可有效缓解大鼠肾组织形态，增强 DN 大鼠肾组织中蛋白的表达，减少肾组织纤维化和炎症浸润。
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