环状RNA circ-PDCD11在肺大细胞癌中高表达并预示着较差的生存率。
CircRNA circ-PDCD11 is highly expressed in lung large-cell carcinoma and predicts poor survival.
作者信息
Ma Tinghang, Miao Hui, Xiong Yongzhong, Ma Yu, Dong Zhen
机构信息
Cancer Center, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan City, Shandong Province, P. R. China.
Department of Cadre Health Section, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan City, Shandong Province, P. R. China.
出版信息
Immunopharmacol Immunotoxicol. 2023 Feb;45(1):89-93. doi: 10.1080/08923973.2022.2117628. Epub 2022 Sep 28.
BACKGROUND
Circ-PDCD11 (hsa_circ_0019853, 461 bp) has been characterized as an oncogenic circRNA in breast cancer, while its function in other cancers is unclear. In this study, we explored the role of circ-PDCD11 in lung cancer.
METHODS
Plasma samples were obtained from patients with lung large-cell carcinoma (LLCC, = 40), lung squamous cell carcinoma (LSCC, = 40), lung adenocarcinoma (LA, = 40) and small-cell lung cancer (SCLC, = 40) as well as healthy controls (Control, = 40). Paired tumor and nontumor tissue samples were obtained from all patients. Expression of circ-PDCD11 in these samples was determined by RT-qPCR. The role of plasma circ-PDCD11 in the diagnosis of LLCC was analyzed with ROC curve. A five-year follow-up was performed to analyze the role of plasma circ-PDCD11 in the prognosis of LLCC.
RESULTS
Plasma circ-PDCD11 was specifically upregulated in LLCC but not in other lung cancer types, compared to the controls. Increased circ-PDCD11 expression in tumor tissues compared to nontumor tissues was only observed in LLCC patients but not in other lung cancer types. Increased plasma circ-PDCD11 levels effectively separated LLCC patients from patients with other types of cancers. High plasma circ-PDCD11 levels were closely correlated with poor survival of LLCC patients. Plasma circ-PDCD11 levels were closely correlated with tumor metastasis, but not tumor size of LLCC.
CONCLUSION
CircRNA circ-PDCD11 is highly expressed specifically in LLCC and predicts poor survival.
背景
环状PDCD11（hsa_circ_0019853，461碱基对）已被证实为乳腺癌中的一种致癌环状RNA，但其在其他癌症中的功能尚不清楚。在本研究中，我们探究了环状PDCD11在肺癌中的作用。
方法
收集了40例肺大细胞癌（LLCC）患者、40例肺鳞状细胞癌（LSCC）患者、40例肺腺癌（LA）患者、40例小细胞肺癌（SCLC）患者以及40例健康对照者的血浆样本。从所有患者中获取配对的肿瘤组织和非肿瘤组织样本。通过RT-qPCR检测这些样本中环状PDCD11的表达。用ROC曲线分析血浆环状PDCD11在LLCC诊断中的作用。进行了为期五年的随访，以分析血浆环状PDCD11在LLCC预后中的作用。
结果
与对照组相比，血浆环状PDCD11在LLCC中特异性上调，而在其他类型肺癌中未上调。仅在LLCC患者中观察到肿瘤组织中环状PDCD11表达高于非肿瘤组织，而在其他类型肺癌中未观察到。血浆环状PDCD11水平升高可有效区分LLCC患者与其他类型癌症患者。血浆环状PDCD11水平高与LLCC患者的不良生存密切相关。血浆环状PDCD11水平与LLCC的肿瘤转移密切相关，但与肿瘤大小无关。
结论
环状RNA环状PDCD11在LLCC中特异性高表达，并预示不良生存。
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