Jiamusi University, Heilongjiang 154007, China.
Xiang'an Hospital of Xiamen University, Fujian 361100, China.
Crit Rev Eukaryot Gene Expr. 2022;32(8):69-80. doi: 10.1615/CritRevEukaryotGeneExpr.2022042848.
The problem facing gastric cancer treatment is the uncontrollable prognosis. Long noncoding RNAs (lncRNAs) are the current hotspot for gastric cancer prognostic markers. This study was targeted at determining THUMPD3-AS1 expression in gastric cancer, and then exploring whether THUMPD3-AS1 is associated with prognosis and its role in cancerous cell function. THUMPD3-AS1 expression levels were quantified in human tissues and cell lines. The prognostic biomarker potential of THUMPD3-AS1 was evaluated by Kaplan-Meier and multivariate Cox regression analyses. The biological impact of THUMPD3-AS1 in gastric cancer cells was investigated by WST-1, Tran-swell, and reactive oxygen species (ROS) accumulation assay. The binding between THUMPD3-AS1, miR-1252-3p and CXCL17 was verified by luciferase reporter assay and RNA pulled down assay. THUMPD3-AS1 was significantly decreased in gastric cancer tissues and cells by comparing them with normal ones. THUMPD3-AS1 was related to the advanced TNM stage, lymphatic infiltration, and vascular infiltration. Downregulated THUMPD3-AS1 was associated with reduced 5-year overall survival. Overexpression of THUMPD3-AS1 inhibits proliferation, migration, invasion and ROS accumulation of gastric cancer cells by regulation of miR-1252-3p and CXCL17. THUMPD3-AS1 could be a potent prognostic symbol for patients with gastric cancer. THUMPD3-AS1 provides a therapeutic potential for gastric cancer.
胃癌治疗面临的问题是不可控的预后。长链非编码 RNA（lncRNA）是目前胃癌预后标志物的研究热点。本研究旨在检测胃癌中 THUMPD3-AS1 的表达，然后探讨 THUMPD3-AS1 是否与预后相关及其在癌细胞功能中的作用。在人组织和细胞系中定量检测 THUMPD3-AS1 的表达水平。通过 Kaplan-Meier 和多变量 Cox 回归分析评估 THUMPD3-AS1 的预后生物标志物潜力。通过 WST-1、Transwell 和活性氧（ROS）积累测定法研究 THUMPD3-AS1 在胃癌细胞中的生物学影响。通过荧光素酶报告测定和 RNA 下拉测定验证 THUMPD3-AS1、miR-1252-3p 和 CXCL17 之间的结合。通过比较正常组织和细胞，发现胃癌组织和细胞中 THUMPD3-AS1 显著降低。THUMPD3-AS1 与较晚期的 TNM 分期、淋巴浸润和血管浸润有关。下调 THUMPD3-AS1 与降低 5 年总生存率有关。通过调节 miR-1252-3p 和 CXCL17，过表达 THUMPD3-AS1 可抑制胃癌细胞的增殖、迁移、侵袭和 ROS 积累。THUMPD3-AS1 可能是胃癌患者的一个潜在的预后标志物。THUMPD3-AS1 为胃癌提供了一种治疗潜力。
