Favere Kasper, Van Fraeyenhove Jens, Jacobs Griet, Bosman Matthias, Eens Sander, De Sutter Johan, Miljoen Hielko, Guns Pieter-Jan, De Keulenaer Gilles W, Segers Vincent F M, Heidbuchel Hein
Laboratory of Physiopharmacology, GENCOR, University of Antwerp, Antwerp, Belgium.
Research Group Cardiovascular Diseases, GENCOR, University of Antwerp, Antwerp, Belgium.
Am J Physiol Heart Circ Physiol. 2022 Oct 1;323(4):H763-H773. doi: 10.1152/ajpheart.00337.2022. Epub 2022 Aug 26.
Cardiac arrhythmias are associated with cardiovascular morbidity and mortality. Cardiac electrophysiology studies (EPS) use intracardiac catheter recording and stimulation for profound evaluation of the heart's electrical properties. The main clinical application is investigation and treatment of rhythm disorders. These techniques have been translated to the murine setting to open opportunities for detailed evaluation of the impact of different characteristics (including genetics) and interventions on cardiac electrophysiology and -pathology. Currently, a detailed description of the technique of murine transjugular EPS (which is the standard route of catheter introduction) is lacking. This article provides detailed information on EPS in mice via the transjugular route. This includes catheter placement, stimulation protocols, intracardiac tracing interpretation, artifact reduction, and surface ECG recording. In addition, reference values as obtained in C57BL/6N mice are presented for common electrophysiological parameters. This detailed methodological description aims to increase accessibility and standardization of EPS in mice. Ultimately, also human research and patient care may benefit from translation of the knowledge obtained in preclinical models using this technique. Electrophysiology studies (EPS) allow in-depth evaluation of cardiac electrophysiology and -pathology. These techniques have been adapted to the murine setting for (translational) studies, mainly focusing on arrhythmogenesis. Despite the frequent application of EPS via the transjugular route, a thorough description of the technique is currently lacking. This article aims to function as a comprehensive guide, also elaborating (for the first time) on nonsurgical aspects such as catheter positioning, tracing artifacts, stimulation protocols, and reference values.
心律失常与心血管疾病的发病率和死亡率相关。心脏电生理研究（EPS）采用心内导管记录和刺激来深入评估心脏的电特性。其主要临床应用是心律失常的研究和治疗。这些技术已被应用于小鼠模型，为详细评估不同特征（包括遗传学）和干预措施对心脏电生理及病理的影响提供了机会。目前，缺乏对小鼠经颈静脉EPS技术（这是导管插入的标准途径）的详细描述。本文提供了经颈静脉途径进行小鼠EPS的详细信息。这包括导管放置、刺激方案、心内描记解读、伪迹减少以及体表心电图记录。此外，还给出了C57BL/6N小鼠常见电生理参数的参考值。这一详细的方法学描述旨在提高小鼠EPS的可及性和标准化程度。最终，临床前模型中使用该技术所获得的知识转化也可能使人类研究和患者护理受益。电生理研究（EPS）能够深入评估心脏电生理及病理情况。这些技术已被应用于小鼠模型进行（转化）研究，主要聚焦于心律失常的发生机制。尽管经颈静脉途径的EPS应用频繁，但目前缺乏对该技术的全面描述。本文旨在作为一份全面指南，还首次详细阐述了诸如导管定位、描记伪迹、刺激方案和参考值等非手术方面的内容。
