豚鼠皮质类固醇结合球蛋白的同源放射免疫测定法。

A homologous radioimmunoassay for guinea pig corticosteroid-binding globulin.

作者信息

Hsu B R, Kato E A, Raymoure W J, Kuhn R W

出版信息

Proc Soc Exp Biol Med. 1987 Jul;185(3):243-9. doi: 10.3181/00379727-185-42540.

PMID:3601945

Abstract

A rapid, specific, and sensitive (requiring only 20 fmole of antigen equivalent to 0.007 microliter of serum) radioimmunoassay (RIA) was developed for the measurement of guinea pig corticosteroid-binding globulin (CBG). CBG was purified to homogeneity from guinea pig serum by affinity chromatography and used for immunization, as the standard and as the radiolabeled trace in the RIA. The antiserum to CBG was raised in rabbits. It was judged specific by immunoelectrophoresis and by comparison of RIA values with steroid-binding assay profiles obtained on serum separated on the basis of size and ion-exchange properties. The results of the radioimmunoassays agree with those of a steroid-binding assay run on identical samples. The sensitivity of the assay allows detection of CBG in serial serum samples, other biologic fluids such as milk, and cell culture supernatants.

摘要

已开发出一种快速、特异且灵敏（仅需20飞摩尔抗原，相当于0.007微升血清）的放射免疫分析法（RIA），用于检测豚鼠皮质类固醇结合球蛋白（CBG）。通过亲和层析从豚鼠血清中纯化出均一的CBG，用于免疫、作为标准品以及RIA中的放射性标记微量物质。用兔制备抗CBG血清。通过免疫电泳以及将RIA值与基于大小和离子交换特性分离的血清的类固醇结合分析图谱进行比较，判断其特异性。放射免疫分析结果与相同样品的类固醇结合分析结果一致。该分析方法的灵敏度能够检测连续血清样品、其他生物体液如乳汁以及细胞培养上清液中的CBG。