Głyda A, Kozak W, Waśko R
Instytutu Ginekologii i Połoznictwa Akademii Medycznej, Poznaniu.
Ginekol Pol. 1994 Nov;65(11):642-5.
We used corticosteroid binding globulin purified by chromatography on the Sepharose column for immunisation of the rabbits. We analysed serum by rocket immunoelectrophoresis and cross immunoelectrophoresis. The titre of the serum was estimated by reaction with 125 I-labelled transcortin (corticosteroid binding globulin). The results of our studies indicate that own serum is valuable and can be use for radioimmunoassay of the CBG in the serum.
我们使用通过琼脂糖凝胶柱层析纯化的皮质类固醇结合球蛋白对兔子进行免疫。我们通过火箭免疫电泳和交叉免疫电泳分析血清。通过与¹²⁵I标记的皮质素转运蛋白（皮质类固醇结合球蛋白）反应来估计血清滴度。我们的研究结果表明，自身血清很有价值，可用于血清中CBG的放射免疫测定。
