Ventura U, Carandente F, Montini E, Sforza C, Ceriani T, Iurlaro E, Moggio R
Prog Clin Biol Res. 1987;227A:361-70.
In male Wistar rats standardized from birth in LD 12:12 conditions (with light from 0700 to 1900 hr), samples of mucosa from the body of the stomach were isolated in vitro and mounted in a Ussing-type chamber. Spontaneous secretion of the hydrogen ion (H+)during 45 min of incubation was measured. Some electric parameters [transmucosal potential difference (PD) and electrical resistance (R)] were detected in the gastric mucosa both during H+ secretion and during inhibition of acid secretion by cimetidine. The variables studied were analyzed with the single cosinor method, and all revealed a circadian rhythm with acrophases during the dark span. The higher values of PD and R found during the dark hours are unrelated to acid secretion and may indicate a greater efficiency of ionic pumps and a limited passive permeability.
在从出生起就在12小时光照/12小时黑暗（光照时间为07:00至19:00）条件下标准化饲养的雄性Wistar大鼠中，从胃体分离出胃黏膜样本并在体外安装于尤斯灌流小室中。测量了孵育45分钟期间氢离子（H⁺）的自发分泌情况。在H⁺分泌期间以及西咪替丁抑制胃酸分泌期间，检测了胃黏膜的一些电学参数[跨黏膜电位差（PD）和电阻（R）]。采用单余弦分析法对所研究的变量进行分析，所有结果均显示在黑暗时段有昼夜节律且峰值相位在此期间。黑暗时段发现的较高的PD和R值与胃酸分泌无关，可能表明离子泵效率更高且被动通透性有限。
