Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Department of Anesthesiology, China-Japan Friendship Hospital, Beijing 100029, China.
J Pharm Biomed Anal. 2022 Oct 25;220:115014. doi: 10.1016/j.jpba.2022.115014. Epub 2022 Aug 23.
Rocuronium is widely used in surgery as a neuromuscular relaxant, but it has been difficult to accurately control its specific dosage in clinical operation. Therefore, the development of fast and instant rocuronium detection methods has important application value for reducing risks and safeguarding health. In this study, N, N, N-trimethyl-4-(pyrene-1-butyl)-ammonium bromide (PyBTA) was designed as a probe to detect rocuronium rapidly. The method relied on replacing PyBTA in sugammadex with rocuronium to induce changes in fluorescence intensity of PyBTA, thereby realizing quantitative detection. Its sensing performance and detection mechanism were explored systematically by spectroscopy. The linear range of this method was 0.5-10 μM and the detection limit of it was 0.3 μM. In addition, we confirmed that the host-guest interaction among PyBTA, sugammadex, and rocuronium was mainly driven by electrostatic and hydrophobic interactions.
罗库溴铵在外科手术中被广泛用作神经肌肉松弛剂，但在临床手术中很难准确控制其特定剂量。因此，开发快速即时的罗库溴铵检测方法对于降低风险和保障健康具有重要的应用价值。在本研究中，设计了 N，N，N-三甲基-4-（芘-1-丁基）-溴化铵（PyBTA）作为探针，以快速检测罗库溴铵。该方法依赖于用罗库溴铵取代琥胆酸钠中的 PyBTA，以诱导 PyBTA 荧光强度的变化，从而实现定量检测。通过光谱法系统地研究了该方法的传感性能和检测机制。该方法的线性范围为 0.5-10 μM，检测限为 0.3 μM。此外，我们证实 PyBTA、琥胆酸钠和罗库溴铵之间的主客体相互作用主要由静电和疏水相互作用驱动。
