Li Xiaoxiao, Chang Pan, Liu Xing, Gong Deying, Zhang Wensheng
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.
Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.
Front Vet Sci. 2025 Mar 4;12:1543086. doi: 10.3389/fvets.2025.1543086. eCollection 2025.
Monitoring rocuronium bromide (Rocur) concentrations is crucial for assessing muscle relaxation in clinical anesthesia. However, no suitable instruments are currently available. This study explores the application of a portable mass spectrometer (MS) for the rapid detection of Rocur concentrations in whole blood from beagles, aiming to support the development of individualized pharmacokinetic-pharmacodynamic (PK-PD) models. Four beagles (1-1.5 years old, 8-12 kg) received a single intravenous dose of Rocur (3 ED₅₀, 0.748 mg/kg). Neuromuscular monitoring was conducted using the train of four (TOF) ratio. Blood samples (0.1 mL) were collected at predetermined intervals and during recovery, with TOF ratios recorded at corresponding time points. Rocur concentrations in whole blood (C) were quantified using both the Cell portable MS and high-performance liquid chromatography with mass spectrometry (HPLC-MS) for consistency assessment. Additionally, a PK-PD model was developed based on C measurements obtained from the Cell portable MS. A strong linear relationship was observed for Cell portable MS measurements within the range of 50-10,000 ng/mL ( = 1108.32 * + 14873.99, = 0.993), with a limit of quantification (LOQ) of 50 ng/mL and a limit of detection (LOD) of 10 ng/mL. A strong linear correlation was found between the two techniques ( = 1.07 * + 30.08, < 0.0001, = 0.8948), with a relative standard deviation <15% for all concentrations. The C values were 4.52 ± 1.16 μg/mL (Cell portable MS) and 4.89 ± 0.52 μg/mL (HPLC-MS), respectively. As C decreased, the TOF ratio gradually recovered, with an IC of 0.25 ± 0.05 μg/mL. This study successfully applied the Cell portable MS for rapid quantitative Rocur analysis in whole blood, demonstrating high consistency with HPLC-MS. The findings also revealed the good correlation between the PK-PD properties of Rocur and TOF effects.
监测罗库溴铵（罗库）浓度对于评估临床麻醉中的肌肉松弛情况至关重要。然而，目前尚无合适的仪器。本研究探索了便携式质谱仪（MS）在快速检测比格犬全血中罗库浓度方面的应用，旨在支持个体化药代动力学 - 药效学（PK - PD）模型的开发。四只比格犬（1 - 1.5岁，8 - 12千克）接受了单次静脉注射罗库（3倍半数有效剂量，0.748毫克/千克）。使用四个成串刺激（TOF）比值进行神经肌肉监测。在预定时间间隔和恢复期间采集血样（0.1毫升），并在相应时间点记录TOF比值。使用Cell便携式质谱仪和高效液相色谱 - 质谱联用（HPLC - MS）对全血中的罗库浓度（C）进行定量，以评估一致性。此外，基于从Cell便携式质谱仪获得的C测量值建立了PK - PD模型。在50 - 10,000纳克/毫升范围内，Cell便携式质谱仪测量值呈现出很强的线性关系（ = 1108.32 * + 14873.99， = 0.993），定量限（LOQ）为50纳克/毫升，检测限（LOD）为10纳克/毫升。两种技术之间发现了很强的线性相关性（ = 1.07 * + 30.08， < 0.0001， = 0.8948），所有浓度下的相对标准偏差<15%。C值分别为4.52 ± 1.16微克/毫升（Cell便携式质谱仪）和4.89 ± 0.52微克/毫升（HPLC - MS）。随着C值降低，TOF比值逐渐恢复，半数有效浓度为0.25 ± 0.05微克/毫升。本研究成功将Cell便携式质谱仪应用于全血中罗库的快速定量分析，与HPLC - MS表现出高度一致性。研究结果还揭示了罗库的PK - PD特性与TOF效应之间的良好相关性。
