Université de Poitiers, CHU Poitiers, CNRS 7267 EBI, INSERM CIC 1402, F-86000, Poitiers, France; Pôle Biologie - Pharmacie, Santé Publique, CHU de Poitiers, 2 Rue de la Milétrie, 86021, Poitiers Cedex, France; Faculté de Médecine et Pharmacie, Université de Poitiers, TSA 51115, 86073, Poitiers Cedex, France.
Université de Poitiers, CHU Poitiers, CNRS 7267 EBI, INSERM CIC 1402, F-86000, Poitiers, France; Faculté de Médecine et Pharmacie, Université de Poitiers, TSA 51115, 86073, Poitiers Cedex, France.
Ecotoxicol Environ Saf. 2022 Sep 15;243:113986. doi: 10.1016/j.ecoenv.2022.113986. Epub 2022 Aug 24.
Bisphenols and parabens are endocrine disruptors families widely used in daily life. They are known to be linked to numerous pathologies such as reproductive disorders, obesity, breast cancer, hypertension and asthma. Biomonitoring is an essential tool for assessing population exposure to environmental pollutants. Blood and urine are the main matrices used in human biomonitoring. However, they are not suitable to evaluate long-term exposure to endocrine disruptors with a short elimination half-life such as parabens or phenols. Hair appears to be an interesting alternative matrix allowing a wide window of exposure due to an accumulation of xenobiotics during hair growth. This study presents the development and validation of a high-performance liquid chromatography coupled to tandem mass spectrometry for the simultaneous determination of bisphenol A, its chlorinated derivatives, bisphenol F, bisphenol S and parabens in human hair. An optimised sample preparation based on acidic hydrolysis followed by liquid-liquid extraction was performed, before an analysis by ultra-high performance liquid chromatography coupled to tandem mass spectrometry in multiple reaction monitoring mode. To validate the method, recognized bioanalytical guidelines were used and calibration and quality control samples were prepared in human hair samples. Linearities were over 0.996 in the whole range of concentrations. Trueness and precision were demonstrated for each target analyte with intra-day and inter-day bias values ranging from 86 % to 118 % and relative standard deviation values ranging from 0 % to 19 %. At the same time, limits of quantification were set at 0.25 ng/g for bisphenol A and parabens, 0.05 ng/g for bisphenols F and S and 0.00625 ng/g for the chlorinated derivatives of bisphenol A. This reliable method was applied to hair samples taken from hospital professionals and allowed the quantification of these endocrine disruptors in this population. Chlorinated derivatives of bisphenol A were quantified here in hair for the first time.
双酚类和对羟基苯甲酸酯类是广泛应用于日常生活中的两种内分泌干扰物家族。已知它们与许多疾病有关，如生殖障碍、肥胖、乳腺癌、高血压和哮喘。生物监测是评估人群暴露于环境污染物的重要工具。血液和尿液是人体生物监测中主要使用的基质。然而，它们并不适合评估具有短消除半衰期（如对羟基苯甲酸酯或酚类）的内分泌干扰物的长期暴露情况。头发似乎是一种很有前途的替代基质，由于在头发生长过程中外源化学物质的积累，可以提供一个广泛的暴露窗口。本研究提出了一种高效液相色谱-串联质谱法，用于同时测定人发中的双酚 A、其氯化衍生物、双酚 F、双酚 S 和对羟基苯甲酸酯。采用基于酸性水解和液液萃取的优化样品前处理方法，然后在超高效液相色谱-串联质谱仪中进行多反应监测模式分析。为了验证该方法，采用了公认的生物分析指南，并在人发样品中制备了校准和质控样品。在整个浓度范围内，线性度均大于 0.996。对于每个目标分析物，通过日内和日间偏差值在 86%至 118%之间，相对标准偏差值在 0%至 19%之间，证明了准确度和精密度。同时，将定量限设定为双酚 A 和对羟基苯甲酸酯为 0.25 ng/g，双酚 F 和 S 为 0.05 ng/g，双酚 A 的氯化衍生物为 0.00625 ng/g。该可靠的方法应用于人发样品中，能够定量分析该人群中的这些内分泌干扰物。本研究首次在头发中定量分析了双酚 A 的氯化衍生物。
