Wu Ho-Cheng, Chen Yu-Chang, Hsieh Chin-Lin, Hsiao George, Wang Shih-Wei, Cheng Ming-Jen, Chao Che-Yi, Lee Tzong-Huei, Kuo Yueh-Hsiung
Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, 110, Taiwan; Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, 110, Taiwan.
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan.
Phytochemistry. 2022 Dec;204:113347. doi: 10.1016/j.phytochem.2022.113347. Epub 2022 Aug 24.
One undescribed C terpenoid, calomacroquinoic acid; four undescribed diterpenes, 5α,6α-epoxy-7α-hydroxyferruginol, 15-ethoxysugiol, 7-methoxy-6,7-secoabieta-8,11,13-triene-6,12-diol, and ethyl 7,8-secoabieta-11,14-dioxo-7-ate; two compounds isolated from Nature for the first time, 6β,7α-dihydroxyferruginol and 12-O-methyltaxochinon; and six known compounds were successfully identified from the bark of Taiwan incense cedar Calocedrus formosana. Structures of all isolates were elucidated by physical data (appearance, ultraviolet, infrared, specific rotation, and X-ray) and spectroscopic data (1D and 2D nuclear magnetic resonance, and high-resolution electron ionization mass spectrometry). The biosynthetic pathway of calomacroquinoic acid is also described in the current study. Nitric oxide production in lipopolysaccharide (LPS)-stimulated BV-2 microglia cells was inhibited by 6,7-dehydroferruginol, 7α,11-dihydroxy-12-methoxy-8,11,13-abietriene, and trans-communic acid. Altogether, the bark of C. formosana possessed several potential natural therapeutics against inflammation-related neuronal diseases.
从台湾香杉（Calocedrus formosana）树皮中成功鉴定出一种未描述的倍半萜类化合物——卡洛马奎诺酸；四种未描述的二萜类化合物——5α,6α-环氧-7α-羟基铁锈醇、15-乙氧基苏乔醇、7-甲氧基-6,7-裂环枞-8,11,13-三烯-6,12-二醇以及7,8-裂环枞-11,14-二氧代-7-酸乙酯；两种首次从自然界分离得到的化合物——6β,7α-二羟基铁锈醇和12-O-甲基紫杉醌；以及六种已知化合物。所有分离物的结构均通过物理数据（外观、紫外、红外、比旋光度和X射线）和光谱数据（一维和二维核磁共振以及高分辨电子电离质谱）得以阐明。本研究还描述了卡洛马奎诺酸的生物合成途径。6,7-脱氢铁锈醇、7α,11-二羟基-12-甲氧基-8,11,13-枞三烯以及反式-communic酸可抑制脂多糖（LPS）刺激的BV-2小胶质细胞中一氧化氮的产生。总之，台湾香杉树皮拥有几种针对炎症相关神经疾病的潜在天然治疗药物。
