Franz M, Zahner H, Striebel H P, Langenstrassen L
Trop Med Parasitol. 1987 Mar;38(1):1-7.
Multimammate rats (Mastomys natalensis) infected with Litomosoides carinii were treated 65 days after infection with a single oral dose of 50 mg/kg of CGP 20308 or CGP 20376 (Ciba Geigy Limited, Basel). Autopsies were performed between 1 and 28 days after treatment. The numbers of worms and the numbers and condition of intrauterine developmental stages in the females were determined. Other female filariae were investigated by transmission electron microscopy (TEM). Female filariae were observed to be immobile on Day 2, later became encapsulated, and had almost disappeared on Day 28. The number of embryos had decreased on Day 6 and even more on Day 14, when only 5-10% appeared light-microscopically unaffected. TEM examination of the filarial midbody regions revealed, that the fine structures of all somatic muscle cells began to disintegrate as early as Day 4 after treatment with either drug. The breakdown of these cells was increased on Day 6 and Day 14. The cytoplasm of the nerve fibres was more electron-dense in treated than in untreated worms. No alterations were observed in the hypodermis, the cuticle, the intestine and the uterine epithelia. Sections from anterior and posterior regions of the parasites were studied after treatment with CGP 20308 only. Degenerating nerve fibres were found in the nerve rings of these filariae 4 days after treatment.
感染了卡里尼丝虫（Litomosoides carinii）的多乳鼠（Mastomys natalensis）在感染65天后，单次口服50毫克/千克的CGP 20308或CGP 20376（瑞士巴塞尔汽巴 - 嘉基有限公司）进行治疗。在治疗后1至28天进行解剖。确定了雌虫体内的虫数以及子宫内发育阶段的数量和状况。其他雌虫通过透射电子显微镜（TEM）进行研究。观察到雌虫在第2天就不再活动，随后被包裹起来，到第28天几乎消失。胚胎数量在第6天减少，在第14天减少得更多，此时在光学显微镜下只有5 - 10%的胚胎似乎未受影响。对丝虫虫体中部区域的TEM检查显示，在用任何一种药物治疗后的第4天，所有体肌细胞的精细结构就开始解体。这些细胞的解体在第6天和第14天加剧。与未处理的虫体相比，处理过的虫体神经纤维的细胞质电子密度更高。在皮下组织、角质层、肠道和子宫上皮中未观察到变化。仅在用CGP 20308治疗后，对寄生虫的前部和后部区域进行切片研究。在治疗4天后，在这些丝虫的神经环中发现了退化的神经纤维。
