College of Veterinary Medicine, Gansu Agricultural University, Ying-Men-Cun, Yin-Tan-Lu, An-Ning District, Lanzhou, 730000, Gansu, China.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xu-Jia-Ping, Yan-Chang-Bu, Cheng-Guan District, Lanzhou, 730046, Gansu, China.
Arch Virol. 2022 Nov;167(11):2249-2262. doi: 10.1007/s00705-022-05549-w. Epub 2022 Aug 27.
Porcine deltacoronavirus (PDCoV) is an enteric virus that was first identified in 2012. Although PDCoV has been detected worldwide, there is little information about its circulation in western China. In this study, fecal samples were collected from piglets with watery diarrhea in western China between 2015 and 2018 for the detection of PDCoV. The positive rate was 29.9%. A PDCoV strain (CHN/CQ/BN23/2016, BN23) was isolated and selected for further investigation. Phylogenetic analysis showed that this strain formed an individual cluster between the early Chinese lineage and the Chinese lineage. RDP4 and SimPlot analysis demonstrated that strain BN23 is a recombinant of Thailand/S5015L/2015 and CHN-AH-2004. The pathogenicity of BN23 was evaluated in 3-day-old piglets. Challenged piglets developed serious clinical signs and died at 3 days post-inoculation. Our data show that PDCoV is prevalent in western China and that strain BN23 is highly pathogenic to newborn piglets. Therefore, more attention should be paid to emerging PDCoV strains in western China.
猪德尔塔冠状病毒（PDCoV）是一种肠病毒，于 2012 年首次被发现。虽然 PDCoV 已在全球范围内被检测到，但关于其在中国西部传播的信息却很少。本研究于 2015 年至 2018 年期间，从中国西部出现水样腹泻的仔猪中采集粪便样本，以检测 PDCoV。检测阳性率为 29.9%。分离到一株 PDCoV 毒株（CHN/CQ/BN23/2016，BN23），并选择其进行进一步研究。系统进化分析表明，该分离株在中国早期谱系和中国谱系之间形成了一个单独的聚类。RDP4 和 SimPlot 分析表明，BN23 株是泰国/S5015L/2015 和 CHN-AH-2004 的重组株。在 3 日龄仔猪上评估 BN23 的致病性。攻毒仔猪出现严重的临床症状，并在接种后 3 天死亡。本研究数据表明 PDCoV 在中国西部流行，BN23 株对新生仔猪具有高致病性。因此，应更加关注中国西部新兴的 PDCoV 株。
