Wang Yuanhong, Wang Junna, Zhao You, Liu Yun, Zhang Miao, Deng Xiaoying, Zhu Jie, Li Guoxin, Liu Guangqing
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Research Center of General Administration of Customs, Beijing 100011, China.
Transbound Emerg Dis. 2024 Nov 13;2024:4162458. doi: 10.1155/2024/4162458. eCollection 2024.
Feline coronavirus (FCoV) is an enveloped, positive-sense RNA virus, which is widespread among feline populations, and can cause a fatal serious disease called feline infectious peritonitis (FIP). According to the differences of antigen and genetic composition, FCoV consists of two genotypes, FCoV I and FCoV II. In this study, we have isolated and identified a FCoV I strain named HL2019. Based on the complete genome of HL2019, phylogenetic analysis showed that HL2019 strain formed in the cluster FCoV I which is more closed to human coronavirus 229E (HCoV 229E) and HCoV NL63, while the FCoV I stains is distantly related to FCoV II strains. Analyzing with RDP4 and Simplot software showed that the virus HL2019 is recombinant by the FCoV I China/ZJU1709 and FCoV I Netherlands/UU16 strains. Furthermore, the pathogenicity of HL2019 was evaluated in 9-12-month-old cats. Two of three challenged cats developed serious clinical signs and died at 28-day postchallenge (dpc). Real-time polymerase chain reaction (PCR) analysis showed that HL2019 has broad tissue tropism, especially in the duodenum with viral load up to 10 copies/mg. In summary, our data show that we have successfully isolated a strain of FCoV I named HL2019 that is highly pathogenic to cats.
猫冠状病毒（FCoV）是一种有包膜的正链RNA病毒，广泛存在于猫群中，可引起一种致命的严重疾病，称为猫传染性腹膜炎（FIP）。根据抗原和基因组成的差异，FCoV分为两种基因型，即FCoV I和FCoV II。在本研究中，我们分离并鉴定了一株名为HL2019的FCoV I毒株。基于HL2019的全基因组，系统发育分析表明，HL2019毒株形成于FCoV I簇中，该簇与人类冠状病毒229E（HCoV 229E）和HCoV NL63更为接近，而FCoV I毒株与FCoV II毒株的亲缘关系较远。用RDP4和Simplot软件分析表明，病毒HL2019是由FCoV I中国/ZJU1709株和FCoV I荷兰/UU16株重组而成。此外，我们在9至12月龄的猫中评估了HL2019的致病性。三只受挑战的猫中有两只出现了严重的临床症状，并在攻毒后28天（dpc）死亡。实时聚合酶链反应（PCR）分析表明，HL2019具有广泛的组织嗜性，尤其是在十二指肠，病毒载量高达10拷贝/毫克。总之，我们的数据表明，我们成功分离出了一株对猫具有高致病性的FCoV I毒株HL2019。
