Department of Otolaryngology, The Fourth Medical Center of PLA General Hospital, Beijing 100048, China.
Department of Otorhinolaryngology, Wuhan Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
Cell Mol Biol (Noisy-le-grand). 2022 May 31;68(5):135-140. doi: 10.14715/cmb/2022.68.5.18.
Nowadays, the incidence and mortality of head and neck tumors are gradually increasing. Head and neck malignant tumors (such as laryngeal cancer, hypopharyngeal cancer, oral cancer, nasopharyngeal cancer, oropharyngeal cancer, and other head and neck malignancies) are more common among systemic tumors. The most common pathological head and neck tumor type is squamous cell carcinoma, accounting for about 90%. In this study, immunohistochemical methods were used to collect the normal squamous epithelial tissues of the head and neck, atypical hyperplasia tissues, and head and neck squamous cell carcinomas on a tissue chip for detection. The recombinant LATS1 overexpression plasmid was prepared and transferred into B88 cells. Western blotting, MTT, and Transwell chamber methods were used to detect the effects of LATS1 proliferation, migration, and B88 cell overexpression. The experimental results showed that in head and neck squamous cell carcinoma, the expression of LATS1 protein decreased from 59.3% to 11.3%. At the same time, this protein inhibited the proliferation, migration, and invasion of head and neck squamous epithelial cells and also inhibited epithelium- Interstitial transformation exerts its tumor suppressor effect, indicating that LATS1 may play a tumor suppressor effect as a tumor suppressor gene. An in-depth study of the role and mechanism of LATS1 protein in the occurrence of head and neck squamous cell carcinoma may provide new opportunities for the treatment of head and neck squamous cell carcinoma in the future.
如今，头颈部肿瘤的发病率和死亡率逐渐上升。头颈部恶性肿瘤（如喉癌、下咽癌、口腔癌、鼻咽癌、口咽癌等头颈部恶性肿瘤）在全身肿瘤中较为常见。最常见的头颈部病理肿瘤类型是鳞状细胞癌，约占 90%。本研究采用免疫组织化学方法，在组织芯片上收集头颈部正常鳞状上皮组织、非典型增生组织和头颈部鳞状细胞癌，进行检测。制备重组 LATS1 过表达质粒并转染 B88 细胞，采用 Western blot、MTT、Transwell 小室法检测 LATS1 过表达对 B88 细胞增殖、迁移的影响。实验结果表明，在头颈部鳞状细胞癌中，LATS1 蛋白的表达从 59.3%下降到 11.3%。同时，该蛋白抑制头颈部鳞状上皮细胞的增殖、迁移和侵袭，也抑制上皮间质转化，发挥其抑癌作用，表明 LATS1 可能作为抑癌基因发挥抑癌作用。深入研究 LATS1 蛋白在头颈部鳞状细胞癌发生中的作用和机制，可能为头颈部鳞状细胞癌的治疗提供新的机会。
