Ferdowsi Sevin, Abdolmaleki Arash, Asadi Asadollah, Zahri Saber
Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Bioinformatics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran.
Neurochem Res. 2023 Jan;48(1):161-171. doi: 10.1007/s11064-022-03721-x. Epub 2022 Aug 27.
After a severe peripheral nerve injury, complete functional recovery is rare. Modulating the inflammatory response could be an effective way to enhance peripheral nerve regeneration. The present study aimed to determine the effect of azithromycin on functional recovery following sciatic nerve crush in Wistar rats. 40 male Wistar rats were used in four groups, including: the negative control, sham, and two groups of azithromycin (15 and 150 mg/kg/day) (n = 10).The rats' right sciatic nerve was crushed using a non-serrated clamp. In experimental groups, animals were treated with azithromycin (15 and 150 mg/kg/day) for 7 days. Then, sensory-motor functions were evaluated over eight weeks. Real-time PCR was used to measure the expression of NGF and BDNF genes. At the end of the 4th week, the sensory recovery accelerated in the azithromycin-treated rats so that the reaction times in the groups treated with 15 mg/kg and 150 mg/kg doses of azithromycin reached 5.14 s and 6.61 s, respectively, which were significantly lower than the 12 s in the negative control group (P < 0.05).Eventually, the mean SFI values in the negative control and both azithromycin-treated groups recovered to preoperative levels in the 8th week, with no significant difference between the sciatic lesion groups. Findings showed a seven-day course of azithromycin administered immediately after a sciatic nerve crush could accelerate regeneration and improve motor and sensory function recovery compared to negative controls. These significant effects were observed in both the azithromycin 15 mg/kg and the azithromycin 150 mg/kg treatment groups. Azithromycin treatment upregulated the expression of NGF and BDNF genes in crushed sciatic nerve. Our findings suggest that a seven-day treatment of azithromycin after a sciatic nerve injury could accelerate the regeneration process and improve functional recovery.
严重的周围神经损伤后，完全功能恢复很少见。调节炎症反应可能是促进周围神经再生的有效方法。本研究旨在确定阿奇霉素对Wistar大鼠坐骨神经挤压伤后功能恢复的影响。40只雄性Wistar大鼠被分为四组，包括：阴性对照组、假手术组和两组阿奇霉素组（15和150mg/kg/天）（n = 10）。使用无锯齿夹钳挤压大鼠右侧坐骨神经。在实验组中，动物接受阿奇霉素（15和150mg/kg/天）治疗7天。然后，在八周内评估感觉运动功能。使用实时PCR测量NGF和BDNF基因的表达。在第4周结束时，阿奇霉素治疗的大鼠感觉恢复加快，使得接受15mg/kg和150mg/kg剂量阿奇霉素治疗的组中的反应时间分别达到5.14秒和6.61秒，显著低于阴性对照组的12秒（P < 0.05）。最终，阴性对照组和两个阿奇霉素治疗组的平均SFI值在第8周恢复到术前水平，坐骨损伤组之间无显著差异。研究结果表明，与阴性对照组相比，坐骨神经挤压伤后立即给予七天疗程的阿奇霉素可加速再生并改善运动和感觉功能恢复。在阿奇霉素15mg/kg和阿奇霉素150mg/kg治疗组中均观察到这些显著效果。阿奇霉素治疗上调了挤压坐骨神经中NGF和BDNF基因的表达。我们的研究结果表明，坐骨神经损伤后七天的阿奇霉素治疗可加速再生过程并改善功能恢复。
