Byun Y H, Lee M H, Kim S S, Kim H, Chang H K, Lee T H, Jang M H, Shin M C, Shin M S, Kim C J
Research Institute of Sports Science, Korea University, Seoul, Korea.
J Sports Med Phys Fitness. 2005 Jun;45(2):222-8.
Peripheral nerve injuries are commonly encountered clinical problems and often result in severe functional deficits. In the present study, the effects of treadmill running on the recovery rate of locomotor function and the expression of brain-derived neurotrophic factor (BDNF) mRNA following sciatic crushed nerve injury in rats were investigated.
Comparative investigation was performed over 14 days.
Experimental animal laboratory.
24 male Sprague-Dawley rats weighing 200+/-10 g. Animals were randomly assigned into 3 groups: the sham-operation group, the sciatic nerve injury group, and the sciatic nerve injury and running group.
The right sciatic nerve was crushed for 30 s using a surgical clip. Rats of the running group were made to run on a treadmill for 30 min once a day for 12 consecutive days.
Functional recovery was analyzed using a walking track analysis which can be quantified with the sciatic function index (SFI) and BDNF mRNA expression was analyzed using reverse transcription-polymerase chain reaction (RT-PCR).
Sciatic crushed nerve injury showed characteristic gait changes showing decrease of SFI value and treadmill running significantly enhanced the SFI value. The level of BDNF mRNA expression was increased following sciatic crushed nerve injury and treadmill running significantly suppressed the sciatic nerve injury-induced increment of BDNF mRNA expression.
It can be suggested that treadmill running after peripheral nerve injury is effective in the functional recovery by inhibition on the over-expression of BDNF mRNA.
周围神经损伤是常见的临床问题，常导致严重的功能缺陷。在本研究中，探讨了跑步机跑步对大鼠坐骨神经挤压伤后运动功能恢复率及脑源性神经营养因子（BDNF）mRNA表达的影响。
进行为期14天的对比研究。
实验动物实验室。
24只体重200±10g的雄性Sprague-Dawley大鼠。动物被随机分为3组：假手术组、坐骨神经损伤组和坐骨神经损伤跑步组。
用手术夹将右侧坐骨神经夹闭30秒。跑步组的大鼠每天在跑步机上跑30分钟，连续跑12天。
使用可通过坐骨神经功能指数（SFI）进行量化的行走轨迹分析来分析功能恢复情况，并用逆转录聚合酶链反应（RT-PCR）分析BDNF mRNA表达。
坐骨神经挤压伤显示出特征性的步态变化，表现为SFI值降低，而跑步机跑步显著提高了SFI值。坐骨神经挤压伤后BDNF mRNA表达水平升高，而跑步机跑步显著抑制了坐骨神经损伤诱导的BDNF mRNA表达增加。
可以认为周围神经损伤后进行跑步机跑步通过抑制BDNF mRNA的过度表达对功能恢复有效。
