Laboratoire de Biologie de la Reproduction, Hôpital François Mitterrand, Université de Bourgogne, 14 rue Gaffarel, 21079 Dijon CEDEX, France; and INSERM UMR1231, Université de Bourgogne Franche comté, Dijon, France.
Service de Gynécologie-Obstétrique, Hôpital François Mitterrand, Université de Bourgogne, Dijon, France.
Reprod Fertil Dev. 2022 Sep;34(15):971-979. doi: 10.1071/RD22093.
Recent studies have failed to demonstrate the negative impact of male tobacco smoking on embryo development, raising the question of its actual implication on natural fecundity and assisted reproductive techniques outcomes.
To assess the impact of paternal smoking on embryo development.
In this prospective cohort study, 252 men from couples undergoing in vitro fertilisation (IVF) were included. Each patient was interviewed and took a carbon monoxide breath-test, creating three groups: non-smokers (n =113), former smokers (n =81) and active smokers (n =58). The Top-grade embryo ratio (primary endpoint), embryo morphokinetic parameters and clinical outcomes were assessed.
In a multivariate analyses based on 1521 embryos, no significant difference was found in the top-grade embryo ratio between the groups. Tobacco smoking had no impact on clinical outcomes. Compared to non-smokers the time to the pronuclei fading (tPNf, P =0.006) and the time to the first embryonic cleavage (t2, P =0.002) were shorter in smokers, and the t2 was also slightly shorter in former smokers (P =0.045). No other differences were found in the morphokinetic parameters.
Even if a few differences were observed in the first timing of embryonic events, this study did not highlight a major embryonic and clinical impact of the paternal smoking status.
The results obtained here are reassuring towards IVF outcomes. As maternal smoking is highly controlled in the IVF patients in this study, we speculate that the sperm selection process may limit the adverse effects of tobacco consumption on embryo development.
最近的研究未能表明男性吸烟对胚胎发育的负面影响，这引发了一个问题，即男性吸烟对自然生育力和辅助生殖技术结局的实际影响是什么。
评估父系吸烟对胚胎发育的影响。
在这项前瞻性队列研究中，纳入了 252 名接受体外受精（IVF）的夫妇中的男性。对每位患者进行访谈并进行一氧化碳呼气测试，创建了三个组：不吸烟者（n=113）、前吸烟者（n=81）和现吸烟者（n=58）。评估顶级胚胎比例（主要终点）、胚胎形态动力学参数和临床结局。
在基于 1521 个胚胎的多变量分析中，三组之间顶级胚胎比例没有显著差异。吸烟对临床结局没有影响。与不吸烟者相比，现吸烟者的原核消失时间（tPNf，P=0.006）和第一次胚胎分裂时间（t2，P=0.002）更短，前吸烟者的 t2 也略短（P=0.045）。在形态动力学参数方面没有发现其他差异。
尽管在胚胎事件的最初时间观察到了一些差异，但这项研究并没有突出显示父系吸烟状况对胚胎和临床的重大影响。
这里获得的结果令人安心，因为在这项研究中，IVF 患者的母亲吸烟受到高度控制，我们推测精子选择过程可能限制了烟草消费对胚胎发育的不利影响。
