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一种在蜱虫取食和繁殖过程中具有多种功能的新型库尼茨家族蛋白。

: A novel Kunitz family protein with versatile functions during feeding and reproduction of the tick .

作者信息

Lu Jialin, Wang Kuang, Gao Zhihua, Zhang Songbo, Li Hao, Shi Yanqing, Song Xuecheng, Liu Jingze, Yu Zhijun, Yang Xiaolong

机构信息

Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

出版信息

Front Vet Sci. 2022 Aug 10;9:872244. doi: 10.3389/fvets.2022.872244. eCollection 2022.

DOI:10.3389/fvets.2022.872244
PMID:36032296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9399790/
Abstract

As obligate blood-feeding ectoparasites, ticks secrete a great diversity of antithrombin molecules during feeding. In this study, a novel antithrombin gene named was characterized from the tick . It has an open reading frame size of 426 bp; it encodes 141 amino acids and has a predicted molecular weight of 15.8 kDa. The fibrinogen coagulation test showed that the time of coagulation was increased significantly with increase in rDoenitin-1 protein concentration, and the activated partial thromboplastin time (APTT) and prothrombin time (PT) assays showed that rDoenitin-1 significantly prolonged the coagulation time of APTT, indicating that rDoenitin-1 has an anticoagulant activity . In addition, rDoenitin-1 presents a significant inhibitory activity in thrombin and cathepsin G. The hemolysis rate of rDoenitin-1 in healthy human blood cells was 4.25%, and no obvious hemolysis activity was observed. The comparison with other life stages shows that the higher expression occurs in adults, and tissue comparison indicated a higher expression in the midgut. The RNAi results indicated that interference of significantly reduced the engorgement rate and egg hatchability of , and that the engorged body weight was slightly reduced. In conclusion, the results suggested that the novel gene functions in blood-feeding of and performs various functions during feeding and reproduction of . may be a potential vaccine candidate for tick control and for developing new antithrombotic drugs in the future.

摘要

作为专性吸血的体外寄生虫，蜱在吸血过程中分泌多种抗凝血酶分子。在本研究中，从蜱中鉴定出一个名为的新型抗凝血酶基因。它具有426 bp的开放阅读框大小；编码141个氨基酸，预测分子量为15.8 kDa。纤维蛋白原凝血试验表明，随着重组Doenitin-1蛋白浓度的增加，凝血时间显著延长，活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）测定表明重组Doenitin-1显著延长了APTT的凝血时间，表明重组Doenitin-1具有抗凝血活性。此外，重组Doenitin-1对凝血酶和组织蛋白酶G具有显著的抑制活性。重组Doenitin-1在健康人血细胞中的溶血率为4.25%，未观察到明显的溶血活性。与其他生命阶段的比较表明，成虫中表达较高，组织比较表明中肠中表达较高。RNA干扰结果表明，干扰显著降低了的饱血率和卵孵化率，饱虫体重略有降低。总之，结果表明该新基因在蜱的吸血过程中起作用，并在蜱的取食和繁殖过程中发挥多种功能。可能是未来蜱控制和开发新型抗血栓药物的潜在疫苗候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/ca3d7cfcd32e/fvets-09-872244-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/3831096a139c/fvets-09-872244-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/6db06aee1727/fvets-09-872244-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/d72ab87960b8/fvets-09-872244-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/f8bc4b5ceb11/fvets-09-872244-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/c95828833a68/fvets-09-872244-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/c2df6ca6f30d/fvets-09-872244-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/ca3d7cfcd32e/fvets-09-872244-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/3831096a139c/fvets-09-872244-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/6db06aee1727/fvets-09-872244-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/d72ab87960b8/fvets-09-872244-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/f8bc4b5ceb11/fvets-09-872244-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/c95828833a68/fvets-09-872244-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/c2df6ca6f30d/fvets-09-872244-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77c8/9399790/ca3d7cfcd32e/fvets-09-872244-g0007.jpg

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本文引用的文献

1
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Toxins (Basel). 2021 Dec 20;13(12):913. doi: 10.3390/toxins13120913.
2
Salivary Serpin Iripin-8 Inhibits the Intrinsic Pathway of Coagulation and Complement.唾液丝氨酸蛋白酶抑制剂Iripin-8抑制凝血和补体的内源性途径。
Int J Mol Sci. 2021 Aug 31;22(17):9480. doi: 10.3390/ijms22179480.
3
Insights into the Role of Tick Salivary Protease Inhibitors during Ectoparasite-Host Crosstalk.
浅析蜱唾液蛋白酶抑制剂在寄生虫-宿主串扰中的作用。
Int J Mol Sci. 2021 Jan 17;22(2):892. doi: 10.3390/ijms22020892.
4
The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission.蜱唾液腺和唾液在蜱虫取食及病原体传播中的重要作用
Front Cell Infect Microbiol. 2017 Jun 22;7:281. doi: 10.3389/fcimb.2017.00281. eCollection 2017.
5
Serine Protease Inhibitors in Ticks: An Overview of Their Role in Tick Biology and Tick-Borne Pathogen Transmission.蜱中的丝氨酸蛋白酶抑制剂：其在蜱生物学和蜱传病原体传播中的作用概述
Front Cell Infect Microbiol. 2017 May 22;7:199. doi: 10.3389/fcimb.2017.00199. eCollection 2017.
6
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Thromb Res. 2017 Apr;152:20-29. doi: 10.1016/j.thromres.2017.01.012. Epub 2017 Jan 31.
7
In Vitro Mode of Action and Anti-thrombotic Activity of Boophilin, a Multifunctional Kunitz Protease Inhibitor from the Midgut of a Tick Vector of Babesiosis, Rhipicephalus microplus.嗜牛蜱素的体外作用模式及抗血栓活性，嗜牛蜱素是微小牛蜱（巴贝斯虫病的一种蜱传播媒介）中肠的一种多功能库尼茨蛋白酶抑制剂
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8
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Ticks Tick Borne Dis. 2016 Feb;7(1):180-187. doi: 10.1016/j.ttbdis.2015.09.007. Epub 2015 Oct 22.
9
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PLoS One. 2015 Aug 5;10(8):e0133991. doi: 10.1371/journal.pone.0133991. eCollection 2015.
10
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