Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
Afr Health Sci. 2022 Mar;22(1):673-680. doi: 10.4314/ahs.v22i1.78.
Recently, green nanoparticles are gaining importance in drug development because of their lower toxicity, sustainability, cost effectiveness, simplicity, and ecofriendly nature compared with toxic chemicals.
In this study, we developed a nontoxic method for synthesizing iron oxide nanoparticles by using the fruit of Pouteria caimito that is rich in vitamin A and C and evaluated their cytotoxicity.
Pouteria caimito fruit¬-derived superparamagnetic nanoparticles (PCSNs) were characterized using physical and chemical methods, and their cytotoxicity was examined using the 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) assay.
Ultraviolet-visible spectroscopy (UV-Vis spectro) analysis of PSNs showed a peak at 277 nm. Transmission electron microscopy (TEM) findings showed that PSNs exhibited a nanorod shape with their sizes ranging from 9.41 nm to 16.96 nm (average size: 13.08 nm). The findings of dynamic light scattering (DLS) indicated that the particle size was 186. 6-847.3 d.nm with an average of 367.5 d.nm. The Zeta potential analysis indicated that PSNs exhibited uniform surface charge distribution, and their surface charge was equal to -13.7 mV. Fourier-transform infrared spectroscopy (FTIR) analysis showed that PSNs exhibited bands at 3412, 1629, 1384, 1075, 818, 697, and 471 cm-1. Energy-dispersive X-ray spectroscopy (EDX) results showed that iron was the major element present in PCSNs, followed by other biomolecules such as C, O, and Cl, indicating the production of iron oxide nanoparticles.
The Pouteria caimito fruit that possesses strong oxidizing and nontoxic properties can be a potentially attractive source for the production of iron oxide nanoparticles. Moreover, the cytotoxicity assay results revealed that iron oxide nanoparticles synthesized using the Pouteria caimito fruit extract derived can be used for targeting cancer cells and treating other diseases because of their nontoxic nature. These nanoparticles can be used for the treatment of cancer and other diseases in the future.
与有毒化学物质相比，绿色纳米粒子由于其毒性较低、可持续性、成本效益、简单性和环保性，在药物开发中越来越受到重视。
在这项研究中，我们开发了一种使用富含维生素 A 和 C 的 Pouteria caimito 果实合成氧化铁纳米粒子的无毒方法，并评估了其细胞毒性。
使用物理和化学方法对 Pouteria caimito 果衍生的超顺磁性纳米粒子（PCSNs）进行了表征，并使用 3-（4,5-二甲基噻唑-2-基）-2-5-二苯基四唑溴化铵（MTT）测定法检测了其细胞毒性。
PSNs 的紫外可见光谱（UV-Vis 分光光度法）分析显示在 277nm 处有一个峰。透射电子显微镜（TEM）结果显示，PSNs 呈纳米棒形状，其尺寸范围为 9.41nm 至 16.96nm（平均尺寸：13.08nm）。动态光散射（DLS）的结果表明，粒径为 186.6-847.3 d.nm，平均粒径为 367.5 d.nm。Zeta 电位分析表明，PSNs 表现出均匀的表面电荷分布，其表面电荷等于-13.7mV。傅里叶变换红外光谱（FTIR）分析表明，PSNs 在 3412、1629、1384、1075、818、697 和 471cm-1 处显示出谱带。能量色散 X 射线光谱（EDX）结果表明，PCSNs 中的主要元素是铁，其次是其他生物分子，如 C、O 和 Cl，表明氧化铁纳米粒子的生成。
具有强氧化性和无毒特性的 Pouteria caimito 果实可以成为氧化铁纳米粒子生产的潜在有吸引力的来源。此外，由于其无毒特性，使用 Pouteria caimito 果提取物衍生的铁氧化物纳米粒子合成的细胞毒性测定结果表明，这些纳米粒子可用于靶向癌细胞和治疗其他疾病。这些纳米粒子可用于未来治疗癌症和其他疾病。
