Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
Zabol Medicinal Plants, Research Center, Zabol University of Medical Sciences, Zabol, Iran.
J Biochem Mol Toxicol. 2020 Jun;34(6):e22475. doi: 10.1002/jbt.22475. Epub 2020 Feb 13.
Cerium oxide nanoparticles (CeO NPs) are among the important nanoparticles that are extensively utilized in cosmetics, automotive industries, ultraviolet (UV) filtration, gas sensors, and pharmaceutical products. In this study, CeO NPs were synthesized using an aqueous extract of Ziziphus jujube fruit. The synthesized nanoparticles were characterized using UV-visible spectroscopy, powder X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive spectroscopy, field energy scanning electron microscopy, and Raman methods. The results indicated that the size of synthesized nanoparticles is between 18 and 25 nm, and they have a spherical shape. UV absorbance of the synthesized nanoparticles was measured through spectrophotometric method in the range of 290 to 320 nm. The cytotoxic activity of synthesized CeO NPs against colon (HT-29) cancer cell line was surveyed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The results showed that synthesized nanoparticles are nontoxic on HT-29 cells under 400 μg/mL concentrations after 24 hours of treatment time periods. The increase in treatment time cases increases cytotoxic activity of synthesized nanoparticles. Sun protection factor of CeO NPs, as a criterion for amount of sunlight radiation protection, was determined by applying Mansur equation. The results demonstrated that synthesized CeO NPs have excellent UV protection and sunscreen physical absorption properties.
氧化铈纳米粒子（CeO NPs）是一种重要的纳米粒子，广泛应用于化妆品、汽车工业、紫外线（UV）过滤、气体传感器和制药产品中。在本研究中，使用枣（Ziziphus jujube）果实的水提物合成了 CeO NPs。使用紫外可见光谱、粉末 X 射线衍射、傅里叶变换红外光谱、能谱、场发射扫描电子显微镜和拉曼方法对合成的纳米粒子进行了表征。结果表明，合成纳米粒子的尺寸在 18 至 25nm 之间，呈球形。通过分光光度法在 290 至 320nm 的范围内测量了合成纳米粒子的紫外吸收。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法测定了合成的 CeO NPs 对结肠（HT-29）癌细胞系的细胞毒性活性。结果表明，在 24 小时的处理时间内，浓度在 400μg/mL 以下时，合成的纳米粒子对 HT-29 细胞没有毒性。随着处理时间的增加，合成纳米粒子的细胞毒性活性增加。CeO NPs 的防晒系数（SPF）作为衡量阳光辐射保护程度的标准，通过应用 Mansur 方程来确定。结果表明，合成的 CeO NPs 具有优异的紫外线保护和防晒物理吸收性能。
