Wang Qiao Feng, Liang Li Jun, Sun Jiang Bing, Zhou Jun
Medical School, Xi'an Peihua University, Xi'an, Shaanxi, 710199, China.
Department of Pharmacy, South China Hospital of Shenzhen University, Shenzhen Guangdong 518116, China.
Heliyon. 2022 Aug 10;8(8):e10152. doi: 10.1016/j.heliyon.2022.e10152. eCollection 2022 Aug.
A simple and efficient sample extraction and preconcentration method based on reversed-phase ionic liquid dispersive liquid-liquid microextraction (RP-IL-DLLME) had been developed and used to quantify the domoic acid in human urine samples. The analysis was performed by ultra-performance liquid chromatography and photodiode array detection. During the procedure, hydrophilic ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate [Cmim] BF as dispersive solvent and NaOH solution was chosen as extraction solvent. Some important parameters in the method were investigated to get high enrichment factors. Under optimal conditions, the linearity of the method was in the range of 0.1-10 ng mL and the correlation coefficient was above 0.9996. The relative standard deviations (RSDs) of the developed methods for intra-day () and inter-day () precision ranged from 1.9 to 3.9%. Meanwhile, limit of detection (LOD) was 0.03 ng mL( = 3) and that of quantification (LOQ) was 0.1 ng mL( = 10) with the enrichment factors () being 230. Eventually, the proposed method was successfully applied to the determination of Dominic acid in human urine samples.
基于反相离子液体分散液液微萃取（RP-IL-DLLME）开发了一种简单高效的样品萃取和预浓缩方法，并用于定量测定人尿液样本中的软骨藻酸。采用超高效液相色谱和光电二极管阵列检测进行分析。在此过程中，选择亲水性离子液体1-丁基-3-甲基咪唑四氟硼酸盐[Cmim]BF作为分散溶剂，NaOH溶液作为萃取溶剂。研究了该方法中的一些重要参数以获得高富集因子。在最佳条件下，该方法的线性范围为0.1-10 ng/mL，相关系数高于0.9996。所建立方法的日内（）和日间（）精密度的相对标准偏差（RSD）范围为1.9%至3.9%。同时，检测限（LOD）为0.03 ng/mL（=3），定量限（LOQ）为0.1 ng/mL（=10），富集因子（）为230。最终，该方法成功应用于人尿液样本中软骨藻酸的测定。
