Shum Sara, Kirkwood Jay S, Jing Jing, Petroff Rebekah, Crouthamel Brenda, Grant Kimberly S, Burbacher Thomas M, Nelson Wendel L, Isoherranen Nina
Department of Pharmaceutics, Department of Environmental and Occupational Health Sciences, Washington National Primate Research Center, Center on Human Development and Disability, and Department of Medicinal Chemistry, University of Washington, Health Sciences Building, 1959 NE Pacific Street, Seattle, Washington 98195, United States.
ACS Omega. 2018 Sep 30;3(9):12079-12088. doi: 10.1021/acsomega.8b02115. Epub 2018 Sep 27.
Domoic acid (DA) is a marine neurotoxin produced by several species of . DA causes severe neurological toxicity in humans and animals. To address the current analytical need to quantify low levels of DA in human and animal body fluids, a sensitive and selective high performance liquid chromatography-tandem mass spectrometry method was developed to measure DA in plasma and urine. This method was fully validated to accurately and precisely quantify DA between 0.31 and 16 ng/mL in plasma and between 7.8 and 1000 ng/mL in urine. Our group introduced the use of a novel internal standard, tetrahydrodomoic acid to control for matrix effects and other sources of variability. This validated method will be useful to assess DA concentrations in biological samples of human or animal origin after suspected DA exposure from contaminated food. It will also be applicable to sentinel programs and research studies to analyze body fluids with low levels of DA.
软骨藻酸（DA）是由几种……产生的一种海洋神经毒素。DA会对人类和动物造成严重的神经毒性。为满足当前对人体和动物体液中低水平DA进行定量分析的需求，开发了一种灵敏且具选择性的高效液相色谱-串联质谱法来测定血浆和尿液中的DA。该方法经过全面验证，能够准确且精确地定量血浆中0.31至16 ng/mL以及尿液中7.8至1000 ng/mL的DA。我们团队引入了一种新型内标物——四氢软骨藻酸，以控制基质效应和其他变异性来源。这种经过验证的方法将有助于评估在怀疑因食用受污染食物而接触DA后，人类或动物来源生物样品中的DA浓度。它也将适用于哨兵计划和研究，以分析低水平DA的体液。
