Mallick Niharika, Jha Shailendra K, Agarwal Priyanka, Kumar Sachin, Mall Anchal, M Niranjana, Choudhary Manish K, Chandra Ajay Kumar, Bansal Shreshtha, Saharan M S, Sharma J B
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Department of Genetics and Tree Propagation, Forest Research Institute, Dehradun, India.
Front Genet. 2022 Aug 11;13:941287. doi: 10.3389/fgene.2022.941287. eCollection 2022.
A marker-assisted backcrossing program initiated to transfer leaf rust resistance gene from cv. Trinakria to hexaploid wheat variety HD2932 cotransferred a stripe rust resistance gene, , along with The cross of hexaploid recurrent parent HD2932 with tetraploid donor parent Trinakria produced pentaploid F plants. Fs were backcrossed with recurrent parent HD2932 to produce BCF generation. Foreground and background selection was conducted in each backcross generation to identify plants for backcrossing or selfing. While foreground selection for was carried out with linked and validated molecular marker , for background selection, 86 polymorphic SSR markers from the A and B genomes were used. Single selected plants from BCF and BCF generations backcrossed and selfed to produce BCFand BCF generations, respectively. Background selection resulted in 83.72%, 91.86%, and 98.25% of RPG recovery in BCF, BCF, and BCF generations, respectively. A total of 27 plants with in homozygous state were identified in BCF generation and selfed to produce 27 BCF NILs. All the NILs were tested for leaf and stripe rust resistance at the seedling stage using seven and one f.sp. rust pathotypes. All the 27 NILs were found to be resistant to both leaf and stripe rust pathotypes. So, these NILs are designated to carry leaf and stripe rust resistance genes .
启动了一个标记辅助回交程序，将叶锈病抗性基因从cv. Trinakria转移到六倍体小麦品种HD2932，同时共转移了一个条锈病抗性基因。六倍体轮回亲本HD2932与四倍体供体亲本Trinakria杂交产生了五倍体F植株。F植株与轮回亲本HD2932回交产生BCF代。在每个回交世代进行前景和背景选择，以鉴定用于回交或自交的植株。在利用连锁且经验证的分子标记进行前景选择时，针对 ，对于背景选择，使用了来自A和B基因组的86个多态性SSR标记。从BCF和BCF代中挑选出的单株分别进行回交和自交，以产生BCF和BCF代。背景选择在BCF、BCF和BCF代中分别使轮回亲本基因组（RPG）恢复率达到83.72%、91.86%和98.25%。在BCF代中总共鉴定出27株处于纯合状态的 植株，并使其自交产生27个BCF近等基因系。在幼苗期使用7个叶锈菌生理小种和1个条锈菌生理小种对所有近等基因系进行叶锈病和条锈病抗性测试。发现所有27个近等基因系对叶锈病和条锈病生理小种均具有抗性。因此，这些近等基因系被指定携带叶锈病和条锈病抗性基因 。
