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一个高质量的基因组组装……（原文不完整，翻译至此）

A High-Quality Genome Assembly of .

作者信息

Chen Yu, Zhang Yongbai, Wang Hongjie, Sun Juan, Ma Lichao, Miao Fuhong, Zhang Zixin, Cheng Yang, Huang Jianwei, Yang Guofeng, Wang Zengyu

机构信息

College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, Qingdao Agricultural University, Qingdao, China.

出版信息

Front Genet. 2022 Aug 12;13:844385. doi: 10.3389/fgene.2022.844385. eCollection 2022.

DOI:10.3389/fgene.2022.844385
PMID:36035157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9412107/
Abstract

Sweet sorghum () is a high-quality bio-energy crop that also serves as food for humans and animals. However, there is little information on the genomic characteristics of . In this study, we presented a high-quality assembly of with PacBio long reads, Illumina short reads, high-throughput chromosome capture technology (Hi-C) sequencing data, gene annotation, and a comparative genome analysis. The results showed that the genome of was assembled to 777 Mb with a contig N50 of 553.47 kb and a scaffold N50 of 727.11 kb. In addition, the gene annotation predicted 37,971 genes and 39,937 transcripts in the genome of . A Venn analysis revealed a set of 7,988 common gene annotations by integrating five databases. A Cafe software analysis showed that 191 gene families were significantly expanded, while 3,794 were significantly contracted in . A GO enrichment analysis showed that the expanded gene families were primarily clustered in the metabolic process, DNA reconstruction, and DNA binding among others. The high-quality genome map constructed in this study provides a biological basis for the future analysis of the biological characteristics of , which is crucial for its breeding.

摘要

甜高粱（）是一种优质的生物能源作物，也可作为人类和动物的食物。然而，关于甜高粱基因组特征的信息却很少。在本研究中，我们利用PacBio长读长、Illumina短读长、高通量染色体捕获技术（Hi-C）测序数据、基因注释以及比较基因组分析，展示了甜高粱的高质量基因组组装。结果表明，甜高粱基因组组装大小为777 Mb，重叠群N50为553.47 kb，支架N50为727.11 kb。此外，基因注释预测甜高粱基因组中有37,971个基因和39,937个转录本。通过整合五个数据库的Venn分析揭示了一组7,988个共同的基因注释。Cafe软件分析表明，甜高粱中有191个基因家族显著扩张，而3,794个基因家族显著收缩。基因本体富集分析表明，扩张的基因家族主要聚集在代谢过程、DNA重建和DNA结合等方面。本研究构建的高质量基因组图谱为未来分析甜高粱的生物学特性提供了生物学基础，这对其育种至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/dc9eb8c69ef8/fgene-13-844385-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/597d06771a6f/fgene-13-844385-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/10b897584efd/fgene-13-844385-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/7ea25156450f/fgene-13-844385-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/3e5d5e610a9b/fgene-13-844385-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/78f6bb79090a/fgene-13-844385-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/dc9eb8c69ef8/fgene-13-844385-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/597d06771a6f/fgene-13-844385-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/10b897584efd/fgene-13-844385-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/7ea25156450f/fgene-13-844385-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/3e5d5e610a9b/fgene-13-844385-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/78f6bb79090a/fgene-13-844385-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd0c/9412107/dc9eb8c69ef8/fgene-13-844385-g006.jpg

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