Li Ying, Zhang Shuqin, Zhang Deqiang, Li Xueping, Gao Zhimin, Jiang Zehui
National State Forestry and Grassland Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing, China.
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Front Genet. 2022 Aug 12;13:893956. doi: 10.3389/fgene.2022.893956. eCollection 2022.
miR166s play an important role in plant tissue differentiation. However, the functions of miR166s in the differentiation of vascular tissue in bamboo have not yet been elucidated. Here, we showed that five miR166s are overexpressed (tags per million reads > 2,000) in underground shoot samples of wild-type (WT) Moso bamboo () and a thick-walled variant ( "Pachyloen") throughout the developmental process. Potential targets of these miR166s include some genes encoding homeodomain-leucine zipper (HD-ZIP) transcription factors and protein kinases. Cleavage sites for miR166s were identified in seven homologs and a protein kinase gene degradome sequencing ( < 0.05). Dual-luciferase and transient expression assays confirmed the binding of miR166s to . Fluorescence hybridization revealed that miR166s were localized to the xylem of the leaf, root, and internode of 2-month-old pot seedlings of WT Moso bamboo. Overall, these findings reveal that miR166s are regulators of vascular tissue differentiation in bamboo. The miR166s identified in our study provide novel targets for bamboo breeding.
miR166s在植物组织分化中发挥重要作用。然而，miR166s在竹子维管组织分化中的功能尚未阐明。在此，我们发现5个miR166s在野生型毛竹（）和厚壁变异体（“Pachyloen”）的地下笋样本整个发育过程中均过表达（每百万 reads数>2000）。这些miR166s的潜在靶标包括一些编码同源异型域-亮氨酸拉链（HD-ZIP）转录因子和蛋白激酶的基因。通过降解组测序在7个同源基因和1个蛋白激酶基因中鉴定出miR166s的切割位点（<0.05）。双荧光素酶和瞬时表达分析证实了miR166s与的结合。荧光原位杂交显示miR166s定位于2月龄盆栽野生型毛竹幼苗叶片、根和节间的木质部。总体而言，这些发现揭示了miR166s是竹子维管组织分化的调节因子。我们研究中鉴定出的miR166s为竹子育种提供了新的靶点。
