Zhang Ting, Xu Dongpu, Lv Guohua, Wang Anqi, Wen Haibo
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
Front Physiol. 2022 Aug 11;13:952744. doi: 10.3389/fphys.2022.952744. eCollection 2022.
() is an important freshwater economy shellfish in China, but it often suffers from air exposure during transportation. In this study, we investigated the histological, physiological (mainly including respiratory metabolism, antioxidant capacity, and immune function), and transcriptomic responses of hepatopancreas in to different times of air exposure. At histological level, air exposure caused vacuolation of digestive cells (24-96 h) and enlargement of digestive tubule lumen (6-96 h) in hepatopancreas. At physiological level, the activities of enzymes related to glycolysis (hexokinase and pyruvate kinase) and anaerobic respiration (lactate dehydrogenase) were increased first (6-24 h) of air exposure, then came back to normal level or even decreased. The activity of aerobic respiration-related enzyme (succinic dehydrogenase) began to reduce from 24 h of air exposure. The activities of antioxidant enzymes (superoxide dismutase and catalase) were enhanced during 6-48 h of air exposure and then returned to control level or even inhibited. The content of malondialdehyde (MDA) increased from 96 h of air exposure. The activities of immune-related enzymes (acid phosphatase and alkaline phosphatase) increased during 6-48 h, then returned to normal or began to decline. At transcriptome level, 44 differentially expressed genes (DEGs) in the hepatopancreas were identified after 96-h air exposure. Among these DEGs, 8 were associated with glycolysis, TCA cycle, immune, and antioxidant, and were downregulated after 96-h air exposure. Taken together, these findings illuminated the response of to air exposure at histological, physiological, and transcriptomic levels, which will be beneficial to the aquaculture and transportation of .
（该物种）是中国重要的淡水经济贝类，但在运输过程中经常遭受空气暴露。在本研究中，我们调查了该物种肝胰腺在不同时长空气暴露下的组织学、生理学（主要包括呼吸代谢、抗氧化能力和免疫功能）及转录组反应。在组织学水平上，空气暴露导致肝胰腺消化细胞空泡化（24 - 96小时）和消化小管腔扩大（6 - 96小时）。在生理学水平上，与糖酵解（己糖激酶和丙酮酸激酶）和无氧呼吸（乳酸脱氢酶）相关的酶活性在空气暴露初期（6 - 24小时）先升高，然后恢复到正常水平甚至下降。与有氧呼吸相关的酶（琥珀酸脱氢酶）活性从空气暴露24小时开始降低。抗氧化酶（超氧化物歧化酶和过氧化氢酶）的活性在空气暴露6 - 48小时内增强，然后恢复到对照水平甚至受到抑制。丙二醛（MDA）含量从空气暴露96小时开始增加。与免疫相关的酶（酸性磷酸酶和碱性磷酸酶）活性在6 - 48小时内升高，然后恢复正常或开始下降。在转录组水平上，空气暴露96小时后，肝胰腺中鉴定出44个差异表达基因（DEGs）。在这些DEGs中，8个与糖酵解、三羧酸循环、免疫和抗氧化相关，在空气暴露96小时后下调。综上所述，这些发现揭示了该物种在组织学、生理学和转录组水平对空气暴露的反应，这将有利于该物种的水产养殖和运输。
