Zhou Wuxian, Jiang Xiaogang, Tan Xuhui, Li Darong, Wang Hua, You Jinwen, Li Xiaoling, Zhang Meide
Key Laboratory of Biology and Cultivation of Herb Medicine, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, China.
Front Plant Sci. 2022 Aug 12;13:972804. doi: 10.3389/fpls.2022.972804. eCollection 2022.
Oliv () is an important Chinese traditional medicinal plant with various health benefits. However, the growth of are seriously affected by continuous cropping, which led to the decrease of the yield and quality. A field experiment was conducted to learn the effects of soil amendments on the growth of under continuous cropping condition, and the biological events which occurred at molecular level were investigated. The results indicated that the content of chlorophyll a (Chl a), chlorophyll b (Chl b), and carotenoid (Car) was significantly higher in SCPM (silicon-calcium-potassium-magnesium fertilizer), SCPMA (SCPM combined with azoxystrobin) and SCPMAOM (SCPM combined with azoxystrobin and organic manure) treatments. Moreover, the yield and the levels of alkaloid, polysaccharide, flavone and total protein in the treatments of SCPM, SCPMA and SCPMAOM were significantly higher than those in the control, and these indexes were all highest in the SCPMAOM treatment. RNA-sequencing (RNA-Seq) is an economical and efficient method to obtain genetic information for species with or without available genome data. In this study, RNA-Seq was performed to understand how continuously cropped responded to the soil amendments at the transcriptome level. The number of differentially expressed genes (DEGs) were as follows: CK vs. SCPM (719 up- and 1456 down-), CK vs. SCPMA (1302 up- and 1748 down-), CK vs. SCPMAOM (1274 up- and 1678 down-). The soil amendments affected the growth of mainly by regulating the genes involved in pathways of 'photosynthesis,' 'plant hormone signal transduction,' 'biosynthesis of unsaturated fatty acids,' 'phenylpropanoid biosynthesis,' and 'starch and sucrose metabolism,' etc. qRT-PCR was performed to validate the expressions of 10 target genes such as , , and , etc., which verified the reliability of RNA-Seq results. Overall, this study revealed the roles and underlying mechanisms of the soil amendments in regulating the growth of continuously cropped at transcriptome level. These findings are beneficial for improving the continuous cropping tolerance and may be valuable for future genetic improvement of .
黄连是一种重要的中国传统药用植物，具有多种健康益处。然而，黄连的生长受到连作的严重影响，导致产量和品质下降。进行了一项田间试验，以了解土壤改良剂对连作条件下黄连生长的影响，并研究了分子水平上发生的生物学事件。结果表明，硅钙钾镁肥（SCPM）、硅钙钾镁肥与嘧菌酯组合（SCPMA）以及硅钙钾镁肥与嘧菌酯和有机肥组合（SCPMAOM）处理下的叶绿素a（Chl a）、叶绿素b（Chl b）和类胡萝卜素（Car）含量显著更高。此外，SCPM、SCPMA和SCPMAOM处理下的产量以及生物碱、多糖、黄酮和总蛋白水平均显著高于对照，且这些指标在SCPMAOM处理中均最高。RNA测序（RNA-Seq）是一种经济有效的方法，可用于获取有或没有可用基因组数据的物种的遗传信息。在本研究中，进行RNA-Seq以了解连作黄连在转录组水平上对土壤改良剂的响应。差异表达基因（DEG）的数量如下：对照与SCPM（719个上调和1456个下调）、对照与SCPMA（1302个上调和1748个下调）、对照与SCPMAOM（1274个上调和1678个下调）。土壤改良剂主要通过调节参与“光合作用”、“植物激素信号转导”、“不饱和脂肪酸生物合成”、“苯丙烷类生物合成”和“淀粉和蔗糖代谢”等途径的基因来影响黄连的生长。进行qRT-PCR以验证如、、等10个靶基因的表达，这证实了RNA-Seq结果的可靠性。总体而言，本研究揭示了土壤改良剂在转录组水平上调节连作黄连生长的作用和潜在机制。这些发现有利于提高连作耐受性，可能对黄连未来的遗传改良具有重要价值。
