College of Plant Sciences & Technology, Huazhong Agricultural University, Wuhan City, Hubei, 430070, PR China; Institute of Chinese Herbal Medicine, Hubei Academy of Agricultural Sciences, Enshi City, Hubei, 445000, PR China.
Institute of Chinese Herbal Medicine, Hubei Academy of Agricultural Sciences, Enshi City, Hubei, 445000, PR China.
Plant Physiol Biochem. 2019 Aug;141:279-290. doi: 10.1016/j.plaphy.2019.06.001. Epub 2019 Jun 5.
Codonopsis tangshen Oliv. (C. tangshen Oliv.), a famous medicinal herb in China, is seriously affected by continuous cropping (C-cro). The physiological and biochemical results indicated that C-cro significantly affected the malonaldehyde (MDA) and chlorophyll content, as well as activities of catalase (CAT) and superoxide dismutase (SOD) when compared with the non-continuous cropping (NC-cro) group. Transcriptome profiling found 762 differentially expressed genes, including 430 up-regulated and 332 down-regulated genes by C-cro. In addition, pathway enrichment analysis revealed that genes related to 'Tyrosine degradation I', 'Glycogen synthesis' and 'Phenylalanine and tyrosine catabolism' were up-regulated, and genes associated with 'Signal transduction', 'Immune system', etc. were down-regulated by C-cro. The expression of target genes was further validated by Q-PCR. In this study, we demonstrated the effects of C-cro on C. tangshen at the transcriptome level, and found possible C-cro responsive candidate genes. These findings could be further beneficial for improving the continuous cropping tolerance.
党参（Codonopsis tangshen Oliv.）是中国著名的药用植物，但它会受到连作障碍的严重影响。与非连作（NC-cro）组相比，生理生化结果表明，连作（C-cro）显著影响了丙二醛（MDA）和叶绿素的含量，以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性。转录组谱分析发现了 762 个差异表达基因，其中 430 个基因上调，332 个基因下调。此外，通路富集分析表明，与“酪氨酸降解 I”、“糖原合成”和“苯丙氨酸和酪氨酸分解代谢”相关的基因上调，而与“信号转导”、“免疫系统”等相关的基因下调。通过 Q-PCR 进一步验证了靶基因的表达。本研究在转录组水平上展示了 C-cro 对党参的影响，并找到了可能的 C-cro 响应候选基因。这些发现可能有助于提高党参的连作耐受性。
