Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, National Engineering Research Center of Sugarcane, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, P. R. China.
Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P. R. China.
PLoS One. 2022 Aug 1;17(8):e0271813. doi: 10.1371/journal.pone.0271813. eCollection 2022.
Codonopsis pilosula subsp. tangshen is one of the most important medicinal herbs used in traditional Chinese medicine. Correct identification of materials from C. pilosula subsp. tangshen is critical to ensure the efficacy and safety of the associated medicines. Traditional DNA molecular markers could distinguish Codonopsis species well, so we need to develop super or specific molecular markers. In this study, we reported the plastome of Codonopsis pilosula subsp. tangshen (Oliv.) D.Y. Hong conducted phylogenomic and comparative analyses in the Codonopsis genus for the first time. The entire length of the Codonopsis pilosula subsp. tangshen plastome was 170,672 bp. There were 108 genes in the plastome, including 76 protein-coding genes, 28 transfer RNA (tRNA), and four ribosomal RNA (rRNA) genes. Comparative analysis indicated that Codonopsis pilosula subsp. tangshen had an unusual large inversion in the large single-copy (LSC) region compared with the other three Codonopsis species. And there were two dispersed repeat sequences at both ends of the inverted regions, which might mediate the generation of this inversion. We found five hypervariable regions among the four Codonopsis species. PCR amplification and Sanger sequencing experiments demonstrated that two hypervariable regions could distinguish three medicinal Codonopsis species. Results obtained from this study will support taxonomic classification, discrimination, and molecular evolutionary studies of Codonopsis species.
党参亚种 Tangshen 是中药中最重要的草药之一。正确识别党参亚种 Tangshen 的材料对于确保相关药物的疗效和安全性至关重要。传统的 DNA 分子标记可以很好地区分党参属物种，因此我们需要开发超级或特定的分子标记。在这项研究中，我们首次报道了党参亚种 Tangshen (Oliv.) D.Y. Hong 的质体基因组，并进行了系统发育和比较分析。党参亚种 Tangshen 的质体全长为 170672bp，其中包含 108 个基因，包括 76 个蛋白编码基因、28 个转移 RNA(tRNA)和 4 个核糖体 RNA(rRNA)基因。比较分析表明，与其他三种党参属物种相比，党参亚种 Tangshen 的大单一拷贝 (LSC) 区域存在一个不寻常的大倒位。并且在倒位区域的两端存在两个分散的重复序列，这可能介导了这个倒位的产生。我们在这四个党参属物种中发现了五个高变区。PCR 扩增和 Sanger 测序实验表明，两个高变区可以区分三种药用党参属物种。本研究的结果将支持党参属物种的分类学分类、鉴别和分子进化研究。
