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Notch信号通路调控应变介导的血管平滑肌细胞表型转换。

Notch signaling regulates strain-mediated phenotypic switching of vascular smooth muscle cells.

作者信息

Karakaya Cansu, van Turnhout Mark C, Visser Valery L, Ristori Tommaso, Bouten Carlijn V C, Sahlgren Cecilia M, Loerakker Sandra

机构信息

Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands.

Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, Netherlands.

出版信息

Front Cell Dev Biol. 2022 Aug 12;10:910503. doi: 10.3389/fcell.2022.910503. eCollection 2022.

DOI:10.3389/fcell.2022.910503
PMID:36036000
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9412035/
Abstract

Mechanical stimuli experienced by vascular smooth muscle cells (VSMCs) and mechanosensitive Notch signaling are important regulators of vascular growth and remodeling. However, the interplay between mechanical cues and Notch signaling, and its contribution to regulate the VSMC phenotype are still unclear. Here, we investigated the role of Notch signaling in regulating strain-mediated changes in VSMC phenotype. Synthetic and contractile VSMCs were cyclically stretched for 48 h to determine the temporal changes in phenotypic features. Different magnitudes of strain were applied to investigate its effect on Notch mechanosensitivity and the phenotypic regulation of VSMCs. In addition, Notch signaling was inhibited DAPT treatment and activated with immobilized Jagged1 ligands to understand the role of Notch on strain-mediated phenotypic changes of VSMCs. Our data demonstrate that cyclic strain induces a decrease in Notch signaling along with a loss of VSMC contractile features. Accordingly, the activation of Notch signaling during cyclic stretching partially rescued the contractile features of VSMCs. These findings demonstrate that Notch signaling has an important role in regulating strain-mediated phenotypic switching of VSMCs.

摘要

血管平滑肌细胞（VSMC）所经历的机械刺激和机械敏感的Notch信号是血管生长和重塑的重要调节因子。然而，机械信号与Notch信号之间的相互作用及其对VSMC表型调节的贡献仍不清楚。在此，我们研究了Notch信号在调节应变介导的VSMC表型变化中的作用。对合成型和收缩型VSMC进行48小时的周期性拉伸，以确定表型特征的时间变化。施加不同幅度的应变以研究其对Notch机械敏感性和VSMC表型调节的影响。此外，通过DAPT处理抑制Notch信号，并使用固定化的Jagged1配体激活Notch信号，以了解Notch在应变介导的VSMC表型变化中的作用。我们的数据表明，周期性应变会导致Notch信号减少，同时VSMC收缩特征丧失。因此，在周期性拉伸过程中激活Notch信号可部分恢复VSMC的收缩特征。这些发现表明，Notch信号在调节应变介导的VSMC表型转换中具有重要作用。

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2
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Biomech Model Mechanobiol. 2022 Feb;21(1):5-54. doi: 10.1007/s10237-021-01521-w. Epub 2021 Oct 6.
3
The Phenotypic Responses of Vascular Smooth Muscle Cells Exposed to Mechanical Cues.
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血管平滑肌细胞对机械刺激的表型反应。
Cells. 2021 Aug 26;10(9):2209. doi: 10.3390/cells10092209.
4
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Arterioscler Thromb Vasc Biol. 2021 Nov;41(11):2693-2707. doi: 10.1161/ATVBAHA.121.316600. Epub 2021 Sep 2.
5
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J Biomed Sci. 2021 May 9;28(1):36. doi: 10.1186/s12929-021-00732-8.
6
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7
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