Shcherbak N S, Suchkova I O, Patkin E L, Voznyuk I A
Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russia.
Institute of Experimental Medicine, St. Petersburg, Russia.
Zh Nevrol Psikhiatr Im S S Korsakova. 2022;122(8. Vyp. 2):32-40. doi: 10.17116/jnevro202212208232.
The enrichment of angioneurology with fundamental advances leads to the understanding of new important facets in the pathogenesis of cerebral ischemia. The knowledge of epigenetic mechanisms in the development of stroke, in particular, DNA methylation, which makes a significant contribution to the development and formation of cerebral damage, is becoming more and more relevant. This review reflects an analysis of animal studies proving the relationship of DNA methylation with cerebral ischemia. As a result of the search work, 282 articles from the PubMed database were selected for keywords that corresponded to this topic. Of these publications, 8 studies were devoted to genome-wide DNA methylation, and 6 published the results of DNA methylation of candidate genes in experimental cerebral ischemia. The results have demonstrated that brain DNA methylation in animals is associated with the development of ischemic stroke and may play a role in several pathogenetic mechanisms. In two studies, a decrease in the level of DNA methylation in 2 genes in ischemic brain tissues of laboratory animals was found, at the same time, in four studies, 8 genes, in which methylation increased after ischemic stroke, were reported. These data suggest that the assessment of the level of DNA methylation in stroke is a promising biomarker for the search and improvement of pharmacological and non-pharmacological methods for limiting brain damage in ischemic and reperfusion injury at the stages of preclinical and clinical studies.
血管神经病学在基础研究方面的进展，有助于我们理解脑缺血发病机制中一些新的重要方面。特别是中风发生过程中的表观遗传机制，尤其是DNA甲基化，它在脑损伤的发生和形成中起着重要作用，这一知识正变得越来越重要。这篇综述反映了对动物研究的分析，这些研究证实了DNA甲基化与脑缺血之间的关系。通过检索工作，从PubMed数据库中筛选出282篇与该主题相关关键词的文章。在这些出版物中，8项研究致力于全基因组DNA甲基化，6项发表了实验性脑缺血中候选基因DNA甲基化的结果。结果表明，动物脑中的DNA甲基化与缺血性中风的发生有关，可能在几种发病机制中起作用。在两项研究中，发现实验动物缺血脑组织中两个基因的DNA甲基化水平降低，同时，在四项研究中，报告了8个基因在缺血性中风后甲基化增加。这些数据表明，在临床前和临床研究阶段，评估中风时DNA甲基化水平是寻找和改进限制缺血和再灌注损伤时脑损伤的药理学和非药理学方法的一个有前景的生物标志物。
