Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, Taiwan.
National Museum of Marine Biology and Aquarium, Pingtung, Taiwan.
Biopreserv Biobank. 2023 Aug;21(4):427-432. doi: 10.1089/bio.2022.0033. Epub 2022 Aug 24.
Vitrification and laser warming have gained popularity over the traditional convective warming techniques in cryopreservation. Laser warming is rapid with uniform effects, thus preventing ice crystal formation in samples. Contemporary laser warming studies have focused on proof-of-concept experiments. Yet, no protocols or techniques have been developed to address the problem of warming samples from long-term storage. Herein, a new approach to laser warming samples without exposing the samples to ambient temperature is introduced. The new device presented has a mean laser-hitting accuracy of 76% ± 16% and a rewarming rate of 59% ± 25% on samples with <1 μL in volume. Although these rates depend on the choice of vitrification solution and mastery of the technique, the approach described represents a successful first step toward laser warming samples from long-term cryo-storage.
玻璃化和激光加热在冷冻保存中已经超越了传统的对流加热技术。激光加热快速且效果均匀，从而防止了样品中冰晶的形成。当前的激光加热研究集中在概念验证实验上。然而，还没有开发出用于解决从长期储存中加热样品的问题的协议或技术。在此，介绍了一种无需将样品暴露于环境温度下即可对其进行激光加热的新方法。所提出的新设备在体积<1 μL 的样品上的平均激光命中率为 76%±16%，重加热率为 59%±25%。尽管这些速率取决于玻璃化溶液的选择和技术的掌握程度，但所描述的方法代表了从长期低温储存中激光加热样品的成功的第一步。
