Lin Chiahsin, Hsieh Wen-Chung, Loeslakwiboon Kanokpron, Huang Cheng-Liang, Chen Ting-Chun, Tsai Sujune
National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan.
Institute of Marine Biology, National Dong Hwa University, Pingtung 94401, Taiwan.
Bioengineering (Basel). 2023 May 18;10(5):605. doi: 10.3390/bioengineering10050605.
Vitrification and ultrarapid laser warming are crucial for the cryopreservation of animal embryos, oocytes, and other cells of medicinal, genetic, and agricultural value. In the present study, we focused on alignment and bonding techniques for a special cryojig that combines a jig tool and jig holder into one piece. This novel cryojig was used to obtain a high laser accuracy of 95% and a successful rewarming rate of 62%. The experimental results indicated that our refined device improved laser accuracy in the warming process after long-term cryo-storage through vitrification. We anticipate that our findings will lead to cryobanking applications that use vitrification and laser nanowarming to preserve cells and tissues from a wide range of species.
玻璃化和超快速激光复温对于动物胚胎、卵母细胞以及其他具有医学、遗传和农业价值的细胞的冷冻保存至关重要。在本研究中，我们专注于一种特殊冷冻夹具的对准和粘结技术，该夹具将夹具工具和夹具支架整合为一体。这种新型冷冻夹具用于获得95%的高激光精度和62%的成功复温率。实验结果表明，我们改进后的装置在长期玻璃化冷冻保存后的复温过程中提高了激光精度。我们预计，我们的研究结果将推动冷冻库应用，利用玻璃化和激光纳米复温技术来保存来自广泛物种的细胞和组织。
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