Department of Chemistry, College of education, University of Al-Qadisiyah, Al-Qadisiyah City, Iraq.
Department of Internal Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah City, Iraq.
Asian Pac J Cancer Prev. 2022 Aug 1;23(8):2617-2621. doi: 10.31557/APJCP.2022.23.8.2617.
The objective of this study was to determine the association between manganese superoxide dismutase (Mn-SOD) gene C47 T polymorphism and the risk of malignant lung cancer in Iraqi smokers.
Blood samples were obtained from 260 lung cancer patients (88 females and 172 males) and 295 healthy individuals (91 females and 204 males). DNA was extracted from blood samples and the SOD2 gene was amplified using specific primers. The nucleotide sequences of the SOD2 gene were analyzed by using BLAST server at National Center for Biotechnology Information (NCBI) and the Raptorx app.
TT, CT, and CC genotypes concentrations were 48.1%, 33.2%, and 18.7%, respectively, in the control group. The concentrations of TT, CT, and CC genotypes were 43.5%, 31.5%, and 25%, respectively, in the case group. There were no statistical differences between cases and controls in terms of genotype frequency of SOD2C47T polymorphism. We observed that SOD2C47T polymorphism CT genotype did not increase the risk of lung cancer development compared to those with TT genotype (OR= 0.951, 95% CI = 0.648-1.396; P = 0.798). In addition, it was observed that CC genotype did not increase the risk of lung cancer development in comparison with TT genotype ( OR=0.673, 95% Cl=0.435-1.041: P=0.075).
These results indicated that there was no association between SOD2C47T polymorphism and the risk of lung cancer development in Iraqi smokers.
本研究旨在探讨锰超氧化物歧化酶（Mn-SOD）基因 C47T 多态性与伊拉克吸烟者肺癌发病风险的关系。
采集 260 例肺癌患者（88 例女性，172 例男性）和 295 例健康对照者（91 例女性，204 例男性）的血样，提取 DNA，采用特异性引物扩增 SOD2 基因，利用 NCBI 的 BLAST 服务器和 Raptorx 应用程序分析 SOD2 基因的核苷酸序列。
对照组 TT、CT 和 CC 基因型的浓度分别为 48.1%、33.2%和 18.7%。病例组 TT、CT 和 CC 基因型的浓度分别为 43.5%、31.5%和 25%。病例组和对照组之间 SOD2C47T 多态性的基因型频率无统计学差异。我们观察到，与 TT 基因型相比，SOD2C47T 多态性 CT 基因型并未增加肺癌发病的风险（OR=0.951，95%CI=0.648-1.396；P=0.798）。此外，与 TT 基因型相比，CC 基因型并未增加肺癌发病的风险（OR=0.673，95%Cl=0.435-1.041；P=0.075）。
这些结果表明，在伊拉克吸烟者中，SOD2C47T 多态性与肺癌发病风险之间无相关性。
