Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510640, China.
Ecotoxicol Environ Saf. 2022 Sep 15;243:114028. doi: 10.1016/j.ecoenv.2022.114028. Epub 2022 Aug 26.
Bactrocera tau (Walker) is a fly pest species mainly distributed in Southeast Asia and the South Pacific; it causes substantial ecological and economic issues because of its destructiveness and rapid reproduction. Chemical sterilization technology can reduce the use of insecticides and is widely applied for insect pest control. In this study, the sterilization efficacy of varying concentrations of four chemosterilants, namely, hexamethylphosphoramide (HMPA), CSII Aqua, 5-fluorouracil (5-FU), and colchicine, on adult pumpkin flies was investigated. The results indicated that a solution of 0.03% HMPA had the highest sterilization efficacy. When the number of sterile males was equal to or exceeded 20 times that of untreated males, the hatching rate of offspring eggs was less than 10%. Chemosterilant treatment significantly altered the levels of acid phosphatase (ACP), alkaline phosphatase (AKP), and B. tau vitellogenin (BtVg); these substances have an important impact on reproductive development. The treatment also decreased the size of the reproductive organs (i.e., testes and ovaries). Our results suggest that 0.03% HMPA has unique sterilization properties and may represent a new chemical agent for the control of B. tau populations in agricultural settings.
瓜实蝇（Bactrocera tau （Walker））是一种蝇类害虫，主要分布在东南亚和南太平洋地区；由于其具有很强的破坏性和快速繁殖能力，因此给生态和经济带来了重大问题。化学绝育技术可以减少杀虫剂的使用，并且被广泛应用于害虫防治。在本研究中，研究人员调查了四种化学绝育剂，即六甲基磷酰胺（HMPA）、CSII Aqua、5-氟尿嘧啶（5-FU）和秋水仙碱，在不同浓度下对成年南瓜蝇的绝育效果。结果表明，0.03%的 HMPA 溶液具有最高的绝育效果。当绝育雄性数量等于或超过未处理雄性数量的 20 倍时，后代卵的孵化率低于 10%。化学绝育处理显著改变了酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和 B. tau 卵黄原蛋白（BtVg）的水平；这些物质对生殖发育有重要影响。处理还降低了生殖器官（即睾丸和卵巢）的大小。我们的研究结果表明，0.03%的 HMPA 具有独特的绝育特性，可能代表一种用于控制农业环境中 B. tau 种群的新型化学药剂。
