Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Food Chem. 2023 Jan 15;399:134005. doi: 10.1016/j.foodchem.2022.134005. Epub 2022 Aug 24.
Mature honeys that brew naturally in the hive develop distinct bioactive components, and thus carry a higher premium due to their superior quality. However, how to identify mature honeys remains difficult. Trace oligosaccharides are a likely source of biomarkers to indicate maturity. Here, we profiled trace oligosaccharides in acacia honey by GC-MS and used a metabolomics strategy to screen oligosaccharides that distinguish honeys with different maturities. Turanose content increased gradually in acacia honey samples and was closely related to the days stored in the hive (p < 0.05). To accurately quantify turanose, a UPLC-ELSD method was developed. Using the established method, honeys with ≥1.20 g/100 g of turanose could be classified as mature acacia honey. Based on the preliminary study, 500 commercial acacia honeys were analyzed, and only 77.2 % of these samples had a satisfactory level of turanose. This work offers a potential method to evaluate the quality of honeys.
天然酿造在蜂巢中的成熟蜂蜜会发展出独特的生物活性成分，因此由于其卓越的品质而具有更高的溢价。然而，如何识别成熟的蜂蜜仍然很困难。痕量低聚糖可能是指示成熟度的生物标志物的来源。在这里，我们通过 GC-MS 对洋槐蜂蜜中的痕量低聚糖进行了分析，并使用代谢组学策略筛选出能区分不同成熟度蜂蜜的低聚糖。棉子糖的含量在洋槐蜂蜜样品中逐渐增加，与在蜂巢中储存的天数密切相关（p<0.05）。为了准确地定量棉子糖，我们开发了一种 UPLC-ELSD 方法。使用所建立的方法，可以将棉子糖含量≥1.20 g/100 g 的蜂蜜分类为成熟的洋槐蜂蜜。基于初步研究，对 500 种商业洋槐蜂蜜进行了分析，只有 77.2%的样品达到了令人满意的棉子糖水平。这项工作提供了一种评估蜂蜜质量的潜在方法。
