利用蛋白核小球藻去除米根霉发酵废水营养耦合生产蛋白饲料。
Rhizopus oryzae fermentation wastewater nutrient removal coupling protein fodder production by employing Chlorella pyrenoidosa.
机构信息
Department of Food Science and Engineering, College of Chemical Engineering, Xiangtan University, Xiangtan, China.
Department of Food Science and Engineering, College of Chemical Engineering, Xiangtan University, Xiangtan, China.
出版信息
Bioresour Technol. 2022 Oct;362:127858. doi: 10.1016/j.biortech.2022.127858. Epub 2022 Aug 28.
The scenario was to investigate feasibilities of employing Chlorella pyrenoidosa for Rhizopus oryzae fermentation wastewater nutrient removal coupling protein fodder production. Results stated that TN, TP, NH-N, COD, BOD removal reached 99.79%, 94.70%, 98.80%, 97.60%, 99.60% to acquire discharge permit under fed-batch manipulation, whilst the peaked protein yield (19.94 g/L) was 6.04-fold more than batch manipulation. Rhizopus oryzae fermentation wastewater feeding C. pyrenoidosa was praised as high-quality protein not only with 26.78% essential amino acids and essential amino acids/nonessential amino acids value of 0.84 but also pathogenic bacteria and heavy metal loads complying with fodder standards. In vitro digestibility of dry matter, protein, lipid, and starch achieving 80.07%, 92.13%, 95.93%, 91.9% and bioavailability of polypeptides, triglycerides, free fatty acids, and oligosaccharides displaying 98.67%, 87.12%, 93.86%, 30.21%, which were roughly-equivalent to corn/soybean fodder. The findings formed exemplifications in utilizing other microalgal systems for wastewater nutrient removal coupling chemicals production.
该方案旨在研究利用蛋白核小球藻去除红曲霉发酵废水营养物并生产蛋白饲料的可行性。结果表明，在分批补料操作下，TN、TP、NH4+-N、COD 和 BOD 的去除率分别达到 99.79%、94.70%、98.80%、97.60%和 99.60%，从而获得了排放标准。而在分批操作下，峰值蛋白产量（19.94 g/L）比分批操作高 6.04 倍。将红曲霉发酵废水喂养蛋白核小球藻被赞誉为高质量蛋白，不仅其必需氨基酸含量为 26.78%，必需氨基酸/非必需氨基酸值为 0.84，而且还符合饲料标准的致病菌和重金属负荷。干物质、蛋白质、脂质和淀粉的体外消化率分别达到 80.07%、92.13%、95.93%和 91.9%，多肽、甘油三酯、游离脂肪酸和低聚糖的生物利用率分别达到 98.67%、87.12%、93.86%和 30.21%，与玉米/大豆饲料大致相当。这些发现为利用其他微藻系统进行废水营养物去除与化学品生产提供了范例。
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