College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Bioresour Technol. 2022 Oct;362:127857. doi: 10.1016/j.biortech.2022.127857. Epub 2022 Aug 26.
In this study, the microbial diversity of size-fractionated anammox sludge in a well-mixed system and their contribution to nitrogen transformation were investigated. Results showed that small granules (0.2-1.0 mm) contributed to the major part of the nitrogen removal rate (56 %) due to its largest mixed liquor volatile suspended solids (1240 ± 80 mg·L). However, large granules (>1.0 mm) possessed the highest relative abundances of Ca. Kuenenia stuttgartiensis and specific anammox activity, representing 49.34 % and 24.45 ± 0.01 mg-N·g-mixed liquor volatile suspended solids·h, respectively. The microbial diversity decreased as the increase of granular size, resulting in microbial community shifting to a simpler model. Metagenomic analysis showed that fine sludge might be the potential major for NO/NO production in the mature well-mixed system under inorganic conditions. This study provides guidance for the evaluation of nitrogen contribution by anammox size-fractionated sludge and the inhibition of the potential NO/NO emission in anammox processes.
在这项研究中，考察了混合系统中不同粒径厌氧氨氧化污泥的微生物多样性及其对氮转化的贡献。结果表明，小颗粒（0.2-1.0 毫米）由于其最大的混合液挥发性悬浮固体（1240±80 毫克/升），对氮去除率（56%）的贡献最大。然而，大颗粒（>1.0 毫米）具有最高的 Ca. Kuenenia stuttgartiensis 相对丰度和特定的厌氧氨氧化活性，分别为 49.34%和 24.45±0.01 毫克氮·克混合液挥发性悬浮固体·小时。随着颗粒尺寸的增加，微生物多样性降低，导致微生物群落向更简单的模型转变。宏基因组分析表明，在无机条件下成熟的混合系统中，细污泥可能是潜在的主要的 NO/NO 产生菌。本研究为评估厌氧氨氧化分形污泥的氮贡献以及抑制厌氧氨氧化过程中潜在的 NO/NO 排放提供了指导。
