Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, PR China.
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, PR China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, PR China.
Fish Shellfish Immunol. 2022 Nov;130:31-42. doi: 10.1016/j.fsi.2022.08.058. Epub 2022 Aug 26.
In general, starch, as a complex carbohydrate, is the most economical energy source in aquaculture for its relatively low cost. However, excessive dietary levels of carbohydrate result in pathological conditions. An 8-week feeding trial with CT (control diet, containing 21% carbohydrate), HC (a high-carbohydrate diet, containing 50% carbohydrate) and HCR (a HC diet supplemented with 0.015% Rhizoma curcumae Longae) was performed to investigate the protective effect of curcumin on high-carbohydrate-induced hepatic oxidative stress and intestine lesion in juvenile Trachinotus ovatus. In the current study, HC group significantly decreased WGR, SGR, plasma CAT activity, intestinal C4 levels, hepatic Nrf2, Keap1, Bach1, HO1, CAT, and GPX mRNA expression as well as ZO-1, Occludin, and Claudin-3, TGF-β mRNA transcription levels, while the opposite was true for plasma AST activity, hepatic MDA contents, intestinal Claudin-15, NF-κB, IL-1β, IL-6, and TNF-α mRNA expression. In contrast with the HC group, the HCR group significantly increased the activities of hepatic CAT, SOD, intestinal C3, C4, IgG and LZM levels, hepatic Nrf2, Bach1, CAT, and GPX mRNA expression as well as intestinal ZO-1, Occludin, Claudin-3, TGF-β and IL-10 mRNA expression levels, but the opposite trend was found in plasma triglyceride content, hepatic lipid deposition, hepatic Keap1 mRNA level as well as intestinal NF-κB, IL-6. In conclusion, high-carbohydrate diet can cause detrimental effect on physiological health status in Trachinotus ovatus, while adding Rhizoma curcumae Longae can improve hepatic and intestinal health status via attenuating the oxidative stress, inflammation, and reducing lipid deposition.
一般来说，淀粉作为一种复杂的碳水化合物，是水产养殖中最经济的能量来源，因为其成本相对较低。然而，过量的碳水化合物饮食会导致病理状况。进行了为期 8 周的 CT（对照饮食，含 21%碳水化合物）、HC（高碳水化合物饮食，含 50%碳水化合物）和 HCR（HC 饮食补充 0.015%姜黄）喂养试验，以研究姜黄素对高脂肪碳水化合物诱导的斜带石斑鱼肝脏氧化应激和肠道损伤的保护作用。在本研究中，HC 组显著降低了 WGR、SGR、血浆 CAT 活性、肠道 C4 水平、肝脏 Nrf2、Keap1、Bach1、HO1、CAT 和 GPX mRNA 表达以及 ZO-1、Occludin 和 Claudin-3、TGF-βmRNA 转录水平，而血浆 AST 活性、肝 MDA 含量、肠道 Claudin-15、NF-κB、IL-1β、IL-6 和 TNF-αmRNA 表达则相反。与 HC 组相比，HCR 组显著提高了肝 CAT、SOD、肠 C3、C4、IgG 和 LZM 水平、肝 Nrf2、Bach1、CAT 和 GPX mRNA 表达以及肠 ZO-1、Occludin、Claudin-3、TGF-β和 IL-10 mRNA 表达水平，但血浆甘油三酯含量、肝脂质沉积、肝 Keap1 mRNA 水平以及肠 NF-κB、IL-6 则呈相反趋势。总之，高脂肪碳水化合物饮食会对斜带石斑鱼的生理健康状况造成不利影响，而添加姜黄可以通过减轻氧化应激、炎症和减少脂质沉积来改善肝脏和肠道健康状况。
